Human polyomaviruses JC and BK in the urine of Brazilian children and adolescents vertically infected by HIV
Human polyomaviruses JC and BK in the urine of Brazilian children and adolescents vertically infected by HIV
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DOI：
10.1590/s0074-02762011000800006
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发表时间：
2011-12-01
期刊：
影响因子：
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通讯作者：
Machado, Clarisse Martins
中科院分区：
文献类型：
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作者：
Machado, Daisy Maria;Fink, Maria Cristina;Machado, Clarisse Martins
本研究的目的是描述BK（BKV）和JC（JCV）人多瘤病毒在人类免疫缺陷病毒（HIV）感染儿童和青少年队列中的尿液排泄特征。共入组了五十六例患者：第一组包括116例HIV感染儿童和青少年[中位年龄= 11.4岁;范围1-22岁];第二组包括40例非HIV感染的健康对照（中位年龄= 11.37岁;范围7-16岁）。在入组时通过聚合酶链反应筛选两组的单个尿液样本中是否存在JCV和BKV DNA。发现JCV和BKV尿排泄的总体率分别为24.4%和40.4%（n = 156）。第I组分别有27.6%和54.3%的受试者尿排泄JCV和BKV。相比之下，第II组17.5%的受试者显示JCV阳性结果，12.5%的受试者显示BKV阳性结果（p Pearson JCV = 0.20; p Pearson BKV < 0.0001）。在第I组中，JCV/BKV脱落与年龄、性别或CD 4值之间无相关性。HIV病毒载量< 50拷贝/mL的患者BKV排泄量较低（p < 0.001），JCV排泄量有降低趋势（p = 0.07）。第I组1例患者（1/116，0.9%）的临床和放射学特征与进行性多灶性白质脑病一致，这表明HIV/多瘤病毒合并感染的儿童应进行监测。
The aim of this study was to characterize the urinary excretion of the BK (BKV) and JC (JCV) human polyomaviruses in a cohort of human immunodeficiency virus (HIV)-infected children and adolescents. One hundred and fifty-six patients were enrolled: Group I included 116 HIV-infected children and adolescents [median age = 11.4 years (y); range 1-22 y]; Group II included 40 non-HIV-infected healthy controls (median age = 11.37 y; range 7-16 y). Single urine samples from both groups were screened for the presence of JCV and BKV DNA by polymerase chain reaction at enrolment. The overall rate of JCV and BKV urinary excretion was found to be 24.4% and 40.4%, respectively (n = 156). Group I had urinary excretion of JCV and BKV in 27.6% and 54.3% of subjects, respectively. In contrast, Group II showed positive results for JCV in 17.5% of subjects and for BKV in 12.5% of subjects (p Pearson JCV = 0.20; p Pearson BKV < 0.0001). In Group I, there was no association between JCV/BKV shedding and age, gender or CD4 values. Patients with an HIV viral load < 50 copies/mL had a lower excretion of BKV (p < 0.001) and a trend of lower JCV excretion (p = 0.07). One patient in Group I (1/116, 0.9%) showed clinical and radiological features consistent with progressive multifocal leukoencephalopathy, suggesting that children with HIV/polyomavirus coinfection should be kept under surveillance.