Testing gene function early in the B cell lineage in mb1-cre mice
Testing gene function early in the B cell lineage in mb1-cre mice
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DOI：
10.1073/pnas.0605944103
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发表时间：
2006-09-12
影响因子：
11.1
通讯作者：
Reth, M.
中科院分区：
文献类型：
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作者：
Hobeika, E.;Thiemann, S.;Reth, M.
mb 1基因编码B细胞抗原受体的Ig-α信号传导亚基，并在骨髓中的极早期前B细胞阶段开始仅在B细胞中表达。我们在这里检查mb 1基因作为cre重组酶在B细胞中表达的宿主位点的功效。我们表明，通过整合到mb 1位点的人源化的cre重组酶，我们获得非常有效的重组loxP位点的B细胞系。从各种报告基因，包括剪接因子SRp 20和DNA甲基化酶Dnmt 1的结果表明，mb 1-cre可能是迄今为止描述的泛B细胞特异性cre表达的最佳模型。在B谱系的早期发育阶段具有有效的cre介导的重组的小鼠系的可用性提供了研究各种基因在B细胞发育和功能中的特异性作用的机会。
The mb1 gene encodes the Ig-alpha signaling subunit of the B cell antigen receptor and is expressed exclusively in B cells beginning at the very early pro-B cell stage in the bone marrow. We examine here the efficacy of the mb1 gene as a host locus for cre recombinase expression in B cells. We show that by integrating a humanized cre recombinase into the mb1 locus we obtain extraordinarily efficient recombination of loxP sites in the B cell lineage. The results from a variety of reporter genes including the splicing factor SRp20 and the DNA methylase Dnmt1 suggest that mb1-cre is probably the best model so far described for pan-B cell-specific cre expression. The availability of a mouse line with efficient cre-mediated recombination at an early developmental stage in the B lineage provides an opportunity to study the role of various genes specifically in B cell development and function.