THE ECOA RESTRICTION AND MODIFICATION SYSTEM OF ESCHERICHIA-COLI 15T- - ENZYME STRUCTURE AND DNA RECOGNITION SEQUENCE
THE ECOA RESTRICTION AND MODIFICATION SYSTEM OF ESCHERICHIA-COLI 15T- - ENZYME STRUCTURE AND DNA RECOGNITION SEQUENCE
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DOI：
10.1002/j.1460-2075.1984.tb01850.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
BICKLE, TA
中科院分区：
文献类型：
--
作者：
SURI, B;SHEPHERD, JCW;BICKLE, TA
E. coli15T-。这是一种不寻常的酶，在功能上与经典的I型限制性内切酶明显相关。基本酶是2-亚基修饰甲基化酶。可以纯化另一种蛋白质种类，其本身没有酶活性，但其将修饰甲基化酶转化为ATP和S-腺苷甲硫氨酸依赖性限制性内切核酸酶。EcoA的DNA识别序列具有与先前确定的I型序列非常相似的总体结构：5“-GAGNNNNNNNGTCA-3”; 3“-CTCNNNNNNNCAGT-5”，其中N可以是任何核苷酸。修饰使特定三核苷酸中的腺苷残基和特定四核苷酸的下链中的腺苷残基甲基化。
The EcoA restriction enzyme from E. coli 15T- was isolated. It is an unusual enzyme, clearly related functionally to the classical type-I restriction enzymes. The basic enzyme is a 2-subunit modification methylase. Another protein species can be purified which by itself has no enzymatic activities but which converts the modification methylase to an ATP and S-adenosylmethionine-dependent restriction endonuclease. DNA recognition sequence of EcoA has an overall structure that is very similar to previously determined type-I sequences: 5''-GAGNNNNNNNGTCA-3''; 3''-CTCNNNNNNNCAGT-5'', where N can be any nucleotide. Modification methylates the adenosyl residue in the specific trinucleotide and the adenosyl residue in the lower strand of the specific tetranucleotide.