MOUSE CORTICAL-CELLS LACKING CELLULAR PRP SURVIVE IN CULTURE WITH A NEUROTOXIC PRP FRAGMENT
MOUSE CORTICAL-CELLS LACKING CELLULAR PRP SURVIVE IN CULTURE WITH A NEUROTOXIC PRP FRAGMENT
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DOI：
10.1097/00001756-199410270-00017
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发表时间：
1994-10-27
期刊：
影响因子：
1.7
通讯作者：
KRETZSCHMAR, HA
中科院分区：
文献类型：
--
作者：
BROWN, DR;HERMS, J;KRETZSCHMAR, HA
为了阐明朊病毒蛋白片段（PrP 106 -126）的神经毒性作用是否以某种方式由朊病毒蛋白（PrPc）的细胞同种型介导，从PrP基因已被破坏的小鼠（PrP 0/0小鼠）制备解离的皮质细胞培养物。培养10天后的细胞存活用MTT测定法进行测试。在正常小鼠的培养物中每隔一天施用PrP 106 -126，持续10天，导致比未处理的细胞多34%的细胞死亡。当PrP 106 -126应用于缺乏PrPc表达的小鼠的培养物时，存活率等于或大于未处理的对照细胞。这些结果支持PrPc的表达是PrP 106 -126的神经毒性作用所必需的这一观点。
To elucidate whether the neurotoxic effect of a prion protein fragment (PrP106-126) is in some way mediated by the cellular isoform of the prion protein (PrPc), dissociated cortical cell cultures were prepared from mice in which the PrP gene had been disrupted (PrP0/0 mice). Cell survival after 10 days in culture was tested with an MTT assay. PrP106-126 applied every second day for 10 days in cultures from normal mice resulted in the death of 34% more cells than in untreated cells. When PrP106-126 was applied to cultures from mice lacking PrPc expression, survival was equal to or greater than that of untreated control cells. These results support the notion that expression of PrPc is required for the neurotoxic effect of PrP106-126.