In Vitro Induction of Adipose-Derived Mesenchymal Stem Cells Differentiated Into Sweat Gland Epithelial Cells
In Vitro Induction of Adipose-Derived Mesenchymal Stem Cells Differentiated Into Sweat Gland Epithelial Cells
复制标题
脂肪间充质干细胞体外诱导分化为汗腺上皮细胞
DOI：
10.1166/jctn.2014.3567
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发表时间：
2014-08
影响因子：
--
通讯作者：
Dahai Hu
中科院分区：
文献类型：
--
作者：
Ke Tao;Xiaozhi Bai;Xiaoqiang Li;Yue Zhang;Jihong Shi;Chaowu Tang;Dahai Hu
方法体外分离、培养脂肪间充质干细胞（ADSCs），诱导其向汗腺上皮细胞（SGECs）分化，为体外构建外分泌汗腺组织提供依据。流式细胞术检测CD 29、CD 34、CD 45、CD 90、CD 105的表达。分别用成骨液和成脂液诱导脂肪干细胞向骨细胞和脂肪细胞分化。用碱性磷酸酶和油红O染色法鉴定骨细胞和脂肪细胞。将培养至第3代的ADSCs用含10%胎牛血清和40 ng/ml肝细胞生长因子（HGF）的DMEM培养液在Matrigel中诱导分化为SGECs。流式细胞仪检测结果显示，CD 29、CD 90、CD 105、CD 34和CD 45的阳性率分别为86.4%、97.7%、89.8%、3.7%和2.3%。定向诱导后的细胞具有脂肪细胞和骨细胞的表型特征。40 ng/mLHGF处理5d后，在Matrigel中可观察到SGECs的分化特征。结论ADSCs可分化为SGECs，为体外构建汗腺组织提供了可能。
ObjectiveIn order to provide basis for the in vitro construction of eccrine sweat gland tissue, we induced the adipose-derived mesenchymal stem cells (ADSCs) to differentiate into sweat gland epithelial cells (SGECs).MethodsADSCs were isolated and cultured in vitro. The expressions of CD29, CD34, CD45, CD90 and CD105 were detected by flow cytometry. ADSCs were induced to differentiate into osteocytes and lipocytes by osteogenic and adipogenic liquid respectively. The obtained osteocytes and lipocytes were identified by alkaline phosphatase and oil red O staining. ADSCs cultured to the third generation were treated with DMEM (10% fetal calf serum and 40 ng/ml hepatocyte growth factor (HGF)) and induced to differentiate into SGECs in matrigel.ResultsThe cultured ADSCs proliferated well with fusiform shape. The detection results by flow cytometry indicated that the positive rates of CD29, CD90, CD105, CD34 and CD45 were 86.4%, 97.7%, 89.8%, 3.7% and 2.3%, respectively. The induced cells presented the phenotypic characteristics of the lipocytes and osteocytes after directed induction. The characteristics of SGECs can be observed in Matrigel 5 days after treated by 40 ng/mL HGF.ConclusionADSCs can differentiate into SGECs which shows the possibility of constructing sweat gland tissue in vitro.