Temperature dependence of DNA translocations through solid-state nanopores.
Temperature dependence of DNA translocations through solid-state nanopores.
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DOI：
10.1088/0957-4484/26/23/234004
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发表时间：
2015-06-12
期刊：
影响因子：
3.5
通讯作者：
Dekker C
中科院分区：
文献类型：
--
作者：
Verschueren DV;Jonsson MP;Dekker C
为了更好地物理理解通过固态纳米孔的 DNA 易位，我们从实验和理论上研究了通过直径 10 nm 氮化硅纳米孔的 λ-DNA 易位的温度依赖性。测得的离子电导 G、DNA 诱导的离子电导阻断 ΔG 和事件频率 Γ 均随着温度的升高而增加，而 DNA 易位时间 τ 则减少。当考虑纳米孔的体电导和表面电导并适当考虑接入电阻时，G 和 ΔG 可以准确描述。发现 DNA 线圈未易位部分上的粘性阻力主导着易位时间的温度依赖性，并且通过扩散和电泳运动之间的平衡很好地描述了事件发生率。在第一个全面的温度研究中，通过固态纳米孔进行 DNA 易位的模型和测量特性之间的良好吻合表明我们的模型捕获了该过程的相关物理原理。
In order to gain a better physical understanding of DNA translocations through solid-state nanopores, we study the temperature dependence of λ-DNA translocations through 10 nm-in-diameter silicon-nitride nanopores, both experimentally and theoretically. The measured ionic conductance G, the DNA-induced ionic-conductance blockades ΔG and the event frequency Γ all increase with increasing temperature while the DNA translocation time τ decreases. G and ΔG are accurately described when bulk and surface conductances of the nanopore are considered and access resistance is incorporated appropriately. Viscous drag on the untranslocated part of the DNA coil is found to dominate the temperature dependence of the translocation times and the event rate is well described by a balance between diffusion and electrophoretic motion. The good fit between modeled and measured properties of DNA translocations through solid-state nanopores in this first comprehensive temperature study, suggest that our model captures the relevant physics of the process.