RanBPM is a novel binding protein for p75NTR
RanBPM is a novel binding protein for p75NTR
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DOI：
10.1016/j.bbrc.2003.08.033
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发表时间：
2003-09-26
影响因子：
3.1
通讯作者：
Huang, BR
中科院分区：
文献类型：
--
作者：
Bai, D;Chen, H;Huang, BR
神经营养蛋白受体 p75NTR 可以在体内和体外诱导信号转导。 p75NTR 转导信号的机制仍然未知。使用酵母双杂交系统，我们鉴定了 Ran 结合蛋白 (Ran-BPM) 与 p75NTR (p75ICD) 胞质内结构域的相互作用蛋白。然后通过哺乳动物细胞中的免疫沉淀和免疫印迹分析来验证这种相互作用。 p75ICD 中与 RanBPM 相互作用的结构域被映射到死亡结构域。 (C) 2003 Elsevier Inc. 保留所有权利。
The neurotrophin receptor p75NTR can induce signal transduction both in vivo and in vitro. The mechanisms by which p75NTR transduces signals have remained mostly unknown. Using yeast two-hybrid system, we identified the Ran-binding protein (Ran-BPM) as an interactor with the intracytoplasmic domain of p75NTR (p75ICD). The interaction was then validated by immunoprecipitation in mammalian cells and immunoblotting analysis. The domain in p75ICD interacting with RanBPM was mapped to the death domain. (C) 2003 Elsevier Inc. All rights reserved.