Analysis of Hepatitis C Virus Superinfection Exclusion by Using Novel Fluorochrome Gene-Tagged Viral Genomes
Analysis of Hepatitis C Virus Superinfection Exclusion by Using Novel Fluorochrome Gene-Tagged Viral Genomes
复制标题
使用新型荧光染料基因标记病毒基因组分析丙型肝炎病毒重复感染排除
DOI：
10.1128/jvi.00871-07
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发表时间：
2007
影响因子：
5.4
通讯作者：
R. Bartenschlager
中科院分区：
文献类型：
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作者：
T. Schaller;Nicole T. Appel;G. Koutsoudakis;S. Kallis;V. Lohmann;T. Pietschmann;R. Bartenschlager
第81卷，no。9, p. 491 - 4603, 2007。第4593页，材料和方法，质粒构建，第4至16行：我们发现在描述荧光色素基因标记的报告质粒的质粒构建时存在错误。我们进行了两次独立的pcr，使用反义底漆Alinker_Xba_Pme_NS5A_aa383 (5 -TTAAACCCAG GTCTAGAACCGCTCGAGGGGGGCTGGCCAAAG-3)和底漆S6660 (5 -CAGGACTGACCACTGACAATC TG-3)或意义上底漆Slinker_Xba_Pme_NS5A_aa383 (5 -TCTAGACCTGGGTTTAAACGTGATGCAGGCTCGTCCA CGGG-3)和反义底漆A7759 (5 -CAGACTCCAGGTCCGGATCTCCAGGC-3)插入的链接器在NS5A氨基酸位置383和反义底漆Alinker_Xba_Pme_NS5A_aa378 (5 -TTAAACCCAGGTCTAGACTG GCCAAAGGTCTTGATGGCCAG-3)和底漆S6660或感觉义引物Slinker_Xba_Pme_NS5A_aa378 （5 -TCT agacctgggtttaaacgtcccccctcgagcggtgatgag -3）和反义引物A7759分别插入NS5A氨基酸378位。扩增子通过第二次PCR组合得到包含完整NS5A编码区和连接子的片段，这些片段被SanDI/RsrII消化并转移到pFKi389LucNS33 _dg_JFH中。对于可能给其他调查人员造成的任何不便，我们深表歉意。
Volume 81, no. 9, p. 4591–4603, 2007. Page 4593, Materials and Methods, Plasmid construction, lines 4 to 16: We have discovered that there is an error in the description of the plasmid construction of fluorochrome gene-tagged reporter plasmids. We performed two separate PCRs, using antisense primer Alinker_Xba_Pme_NS5A_aa383 (5 -TTAAACCCAG GTCTAGAACCGCTCGAGGGGGGCTGGCCAAAG-3 ) and sense primer S6660 (5 -CAGGACTGACCACTGACAATC TG-3 ) or sense primer Slinker_Xba_Pme_NS5A_aa383 (5 -TCTAGACCTGGGTTTAAACGTGATGCAGGCTCGTCCA CGGG-3 ) and antisense primer A7759 (5 -CAGACTCCAGGTCCGGATCTCCAGGC-3 ) for insertion of the linker at NS5A amino acid position 383 and antisense primer Alinker_Xba_Pme_NS5A_aa378 (5 -TTAAACCCAGGTCTAGACTG GCCAAAGGTCTTGATGGCCAG-3 ) and sense primer S6660 or sense primer Slinker_Xba_Pme_NS5A_aa378 (5 -TCT AGACCTGGGTTTAAACGTCCCCCCTCGAGCGGTGATGCAG-3 ) and antisense primer A7759 for insertion at NS5A amino acid position 378, respectively. Amplicons were combined by a second PCR resulting in fragments that comprise the complete NS5A coding region plus linker, which were SanDI/RsrII digested and transferred into pFKi389LucNS33 _dg_JFH. We apologize for any inconvenience this may have caused to other investigators.