Oxygen Transport to Tissue XXX
Oxygen Transport to Tissue XXX
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氧气输送至组织 XXX
DOI：
10.1007/978-0-387-85998-9_20
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Cooper C
中科院分区：
文献类型：
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作者：
Cooper C
癫痫发作对脑血流和代谢的影响已被广泛研究。然而，很少有研究集中在线粒体。我们用近红外光谱法（NIRS）研究了GABA拮抗剂荷包牡丹碱诱导的成年大鼠癫痫发作过程中血红蛋白和细胞色素氧化酶的变化。使用宽带光谱系统，其中光极横跨大鼠头部放置。我们关注荷包牡丹碱添加后的初始癫痫发作，在此期间，氧合血红蛋白（HbO 2）增加，脱氧血红蛋白（HHb）减少，总血红蛋白（Hbtot）增加。线粒体细胞色素氧化酶（oxCCO）的CuA中心氧化后的NIRS信号减弱。在最高荷包牡丹碱剂量（0.25 mg/只动物）下，记录的最大值为：Δ HbO 2 = +19 ± 7 μM; ΔHHb = -12 ± 4 μM; ΔHbtot = +7 ± 4 μM，ΔoxCCO =-1.7 ± 0.3 μM。这些结果与其他NIRS研究基本一致。然而，先前对NADH荧光的测量表明在这些条件下线粒体氧化还原链发生氧化。荷包牡丹碱引起的变化提供了一个有趣的挑战，使用近红外光谱研究线粒体氧化还原状态在体内的物理和生物化学，我们探讨这些明显的异常可能的光谱和/或生化意义。
The effect of seizures on brain blood flow and metabolism has been extensively studied. However, few studies have focused on mitochondria. We used near infrared spectroscopy (NIRS) to study hemoglobin and cytochrome oxidase changes during seizures, induced by the GABA antagonist bicuculline, in the adult rat. A broadband spectroscopy system was used with the optodes placed across the rat head. We focused on the initial seizures post-bicuculline addition during which oxyhemoglobin (HbO2) increased, deoxyhemoglobin (HHb) decreased and total hemoglobin (Hbtot) increased. The NIRS signal associated with the oxidised CuAcentre of mitochondrial cytochromecoxidase (oxCCO) decreased. At the highest bicuculline doses (0.25 mg/animal) the maximum values recorded were: ΔHbO2= +19 ± 7 μM; ΔHHb = -12 ± 4 μM; ΔHbtot = +7 ± 4 μM, ΔoxCCO = - 1.7 ± 0.3 μM. These results are broadly in line with other NIRS studies. However, previous measurements of NADH fluorescence indicateoxidationof the mitochondrial redox chain under these conditions. The changes induced by bicuculline provide an interesting challenge to the physics and biochemistry of using NIRS to study mitochondrial redox states in vivo and we explore the possible spectroscopic and/or biochemical meaning of these apparent anomalies.