Ultrafast Papanicolaou stain. An alternative preparation for fine needle aspiration cytology.
Ultrafast Papanicolaou stain. An alternative preparation for fine needle aspiration cytology.
复制标题
超快巴氏染色剂。
作者：
Grace C H Yang;I. I. Alvarez
本研究的目的是开发一种巴氏染色法，其速度与Diff-Quik一样快，但细胞形态学与ThinPrep处理的细胞形态学一样精细，用于细针抽吸物的最佳评价。经三次改良后获得了满意的结果：（1）用生理盐水再水化风干涂片，（2）使用4%甲醛/65%乙醇固定剂，（3）使用Richard-Allan苏木精2和细胞染色剂。第一个修改恢复了细胞和溶血红细胞的透明度，第二个修改将适当固定和染色所需的时间从几分钟减少到几秒钟，第三个修改简化了程序。这个90秒的方案产生了一个透明的，多色的染色，具有清晰的细胞核和细胞质特征。本方案处理的细胞形态学至少等于（如果不优于）ThinPrep制备的标本的质量，并上级标准巴氏涂片法处理的标本。
The objective of this study was to develop a Papanicolaou stain as fast as Diff-Quik yet with cytomorphology as exquisite as that processed by ThinPrep for the optimal evaluation of fine needle aspirates. Satisfactory results were obtained after three modifications were made: (1) rehydration of air-dried smears with normal saline, (2) use of a 4% formaldehyde/65% ethanol fixative, (3) and use of Richard-Allan Hematoxylin 2 and Cyto-stain. The first modification restored the transparency of the cells and hemolysed red blood cells, the second modification reduced the time needed for proper fixation and staining from minutes to seconds, and the third modification simplified the procedure. This 90-second protocol yields a transparent, polychromatic stain with crisp nuclear and cytoplasmic features. The cytomorphology processed by this protocol is at least equal to, if not better than, the quality of specimens prepared by ThinPrep and superior to those processed by the standard Papanicolaou procedure.