Application of a Filtration- and Isolation-by-Size Technique for the Detection of Circulating Tumor Cells in Cutaneous Melanoma
Application of a Filtration- and Isolation-by-Size Technique for the Detection of Circulating Tumor Cells in Cutaneous Melanoma
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DOI：
10.1038/jid.2010.141
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发表时间：
2010-10-01
影响因子：
6.5
通讯作者：
Massi, Daniela
中科院分区：
文献类型：
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作者：
De Giorgi, Vincenzo;Pinzani, Pamela;Massi, Daniela
对皮肤黑色素瘤患者外周血中的循环肿瘤细胞 (CTC) 的分析提供了有关转移过程的信息，并有可能改善患者的管理。按上皮肿瘤细胞大小分离 (ISET) 是一种直接识别 CTC 的方法，其中由于肿瘤细胞体积较大，因此通过过滤收集肿瘤细胞。迄今为止，ISET仅应用于上皮癌患者的CTC检测，该技术从未应用于皮肤黑色素瘤患者。我们在此通过 ISET 调查了 140 名受试者（87 名皮肤黑色素瘤受试者、10 名接受黑素细胞痣手术的受试者、5 名非黑色素瘤皮肤肿瘤患者和 38 名健康志愿者）外周血中 CTC 的存在。免疫组织化学标记物和实时 RT-PCR 的酪氨酸酶 mRNA 呈阳性，支持将过滤器中捕获的细胞鉴定为 CTC。在对照组和原位黑色素瘤组中均未检测到 CTC。相比之下，29% 的原发性侵袭性黑色素瘤患者和 62.5% 的转移性黑色素瘤患者显示 CTC（P
Analysis of circulating tumor cells (CTC) in the peripheral blood of cutaneous melanoma patients provides information on the metastatic process and potentially improves patient management. The isolation by size of epithelial tumor cells (ISET) is a direct method for CTC identification in which tumor cells are collected by filtration as a result of their large size. So far, ISET has been applied only to CTC detection from epithelial cancer patients, and the technique has never been applied to cutaneous melanoma patients. We herein investigated the presence of CTC by ISET in the peripheral blood of 140 subjects (87 with cutaneous melanomas, 10 subjects undergoing surgery for melanocytic nevi, 5 patients with non-melanoma skin tumors, and 38 healthy volunteers). The identification of the cells trapped in filters as CTC was supported by positivity for immunohistochemical markers and for tyrosinase mRNA by real-time RT-PCR. CTC were neither detected in the controls nor in the in situ melanoma group. In contrast, CTC were shown in 29% of patients with primary invasive melanoma and in 62.5% of metastatic melanoma patients (P