Stage-specific changes in protein phosphorylation accompanying meiotic maturation of mouse oocytes and fertilization of mouse eggs.
Stage-specific changes in protein phosphorylation accompanying meiotic maturation of mouse oocytes and fertilization of mouse eggs.
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伴随小鼠卵母细胞减数分裂成熟和小鼠卵子受精的蛋白质磷酸化的阶段特异性变化。
DOI：
10.1002/jez.1402390311
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Schultz,RM
中科院分区：
文献类型：
--
作者：
Endo,Y;Kopf,GS;Schultz,RM
在小鼠卵母细胞的减数分裂成熟过程中，从生发泡破裂后到中期II和受精后，蛋白质磷酸化模式发生了典型的变化。这些变化发生在体外或体内成熟或受精过程中。观察到总磷蛋白合成的三种主要变化。在第一类中，蛋白质的磷酸化从生发泡阶段开始增加，直到生发泡破裂后，然后在发育到中期II和受精后下降。第二类的特征是成熟过程中蛋白质的磷酸化程度降低，受精后这些蛋白质的磷酸化程度随之升高。第三类的特点是蛋白质的磷酸化在成熟过程中保持相对恒定，但在受精后增加；磷酸酪氨酸磷化蛋白构成主要种类。还检测了分离的生发小泡的放射性标记蛋白和磷蛋白组成，发现一种Mr 29000的磷蛋白仅与生发小泡有关。由于我们以前已经证明了12-O-十四酰佛波醇13-乙酸酯抑制受精(Y.Endo，R.M.Schultz和G.S.Kopf提交)，我们研究了这种化合物对中期II卵子磷蛋白模式的影响。12-O-十四酰佛波醇13-醋酸酯处理可刺激一种特异性磷酸蛋白的磷酸化。
Characteristic changes in the patterns of protein phosphorylation occur during meiotic maturation of mouse oocytes from the time subsequent to germinal vesicle breakdown, through metaphase II, and following fertilization. These changes occur during both in vitro or in vivo maturation or fertilization. Three major classes of changes in total phosphoprotein synthesis are observed. In the first class, protein phosphorylations increase from the germinal vesicle stage until just after germinal vesicle breakdown and then decrease during progression to metaphase II and after fertilization. The second class is characterized by decreases in protein phosphorylation during maturation with subsequent increases in phosphorylation of these proteins after fertilization. The third class is characterized by protein phosphorylations that remain relatively constant during maturation but increase after fertilization; phosphotyrosine phosphoproteins comprise the major species. The radiolabeled protein and phosphoprotein composition of isolated germinal vesicles was also examined, and a phosphoprotein of Mr29,000 is found exclusively associated with the germinal vesicle. Since we have shown previously that 12‐O‐tetradecanoyl phorbol 13‐acetate inhibits fertilization (Y. Endo, R.M. Schultz, and G.S. Kopf, submitted), we examined the effects of this compound on the phosphoprotein patterns of metaphase II eggs. 12‐O‐Tetradecanoyl phorbol 13‐acetate treatment stimulates the phosphorylation of a specific phosphoprotein of Mr80,000.