Expression of ras oncogene p21 protein in normal and neoplastic laryngeal tissues: correlation with histopathological features and epidermal growth factor receptors.
Expression of ras oncogene p21 protein in normal and neoplastic laryngeal tissues: correlation with histopathological features and epidermal growth factor receptors.
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DOI：
10.1038/bjc.1994.195
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发表时间：
1994-06
影响因子：
8.8
通讯作者：
Mancuso, S
中科院分区：
文献类型：
--
作者：
Scambia, G;Catozzi, L;Benedetti Panici, P;Ferrandina, G;Almadori, G;Paludetti, G;Cadoni, G;Distefano, M;Piffanelli, A;Mancuso, S
对7例正常喉粘膜组织和43例喉癌组织进行了p21ras癌基因蛋白的Western blotting分析。在正常喉粘膜(中位数1.94OD，范围0.90~2.17OD)和喉癌(中位数1.74OD，范围0.30~6.37OD)中，p21的表达以光密度(OD)表示。喉癌组织中p21的表达高于正常喉组织(中位数2.54OD，1.76~6.37OD，中位数1.94OD，0.90~2.17OD)(P=0.023)。免疫组织化学分析显示，大多数肿瘤细胞(70%以上)呈免疫染色，而间质成分无反应。P21的表达与肿瘤的部位、分期及组织学分级无关。并探讨ras p21蛋白表达与表皮生长因子受体(EGFR)水平的相关性。EGFR阳性组与阴性组p21表达差异无统计学意义(中位数1.52OD，范围0.30~6.37OD，中位数1.84OD，范围0.93~3.71OD)。我们的研究结果表明，p21蛋白的过度表达与喉癌的恶性表型有关。评估p21蛋白在喉癌中的表达是否具有临床意义，尚需进一步研究。
Western blotting analysis of the p21 ras oncoprotein was performed in seven normal laryngeal mucosa specimens and 43 primary laryngeal cancers. Varying p21 levels, expressed as optical density (OD), were found in normal mucosa (median 1.94 OD, range 0.90-2.17 OD) and in primary laryngeal tumours (median 1.74 OD, range 0.30-6.37 OD). When p21 expression in laryngeal cancer was compared with the normal counterpart, higher levels were found in neoplastic than in normal laryngeal tissue (median 2.54 OD, range 1.76-6.37 OD, vs median 1.94 OD, range 0.90-2.17 OD) (P = 0.023). Immunohistochemical analysis demonstrated that most of the tumour cells (more than 70%) were immunostained while the stromal component was unreactive. No correlation between p21 expression and tumour location, stage and histopathological grade was observed. The correlation between ras p21 protein expression and epidermal growth factor receptor (EGFR) levels was also investigated. EGFR-positive cases did not show any difference in p21 expression with respect to EGFR-negative cases (median 1.52 OD, range 0.30-6.37 OD, vs median 1.84 OD, range 0.93-3.71 OD). Our findings suggest that overexpression of p21 protein is associated with a malignant phenotype in laryngeal cancer. Further studies should be undertaken to evaluate whether the assessment of p21 protein expression may have clinical significance in laryngeal cancer.