Overexpression of a fatty acid amide hydrolase compromises innate immunity in Arabidopsis
Overexpression of a fatty acid amide hydrolase compromises innate immunity in Arabidopsis
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DOI：
10.1111/j.1365-313x.2008.03603.x
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发表时间：
2008-10-01
期刊：
影响因子：
7.2
通讯作者：
Mysore, Kirankumar S.
中科院分区：
文献类型：
--
作者：
Kang, Li;Wang, Yuh-Shuh;Mysore, Kirankumar S.
N-酰基乙醇胺是一组脂质介质，在哺乳动物的各种神经和病理条件下积累。N-酰基乙醇胺信号传导通过多种水解酶的作用而终止，其中脂肪酸酰胺水解酶（FAAH）已得到充分表征。在这里，我们表明，过表达AtFAAH的转基因拟南芥品系更容易受到细菌病原体假单胞菌pv.番茄和番茄疫霉致病变种（P. acetylingae pv.）斑点病。AtFAAH过表达子也对非宿主病原体P. P. P. pv.烟粉虱与野生型相比，AtFAAH过表达子具有较低量的茉莉酸、脱落酸以及游离和共轭水杨酸（SA）。基因表达研究表明，转录的一些植物防御基因，以及参与SA生物合成和信号传导的基因，AtFAAH过表达低于野生型植物。我们的数据表明，FAAH过表达改变了植物激素的积累和信号，这反过来又损害了对细菌病原体的先天免疫。
N-acylethanolamines are a group of lipid mediators that accumulate under a variety of neurological and pathological conditions in mammals. N-acylethanolamine signaling is terminated by the action of diverse hydrolases, among which fatty acid amide hydrolase (FAAH) has been well characterized. Here, we show that transgenic Arabidopsis lines overexpressing an AtFAAH are more susceptible to the bacterial pathogens Pseudomonas syringae pv. tomato and P. syringae pv. maculicola. AtFAAH overexpressors also were highly susceptible to non-host pathogens P. syringae pv. syringae and P. syringae pv. tabaci. AtFAAH overexpressors had lower amounts of jasmonic acid, abscisic acid and both free and conjugated salicylic acid (SA), compared with the wild-type. Gene expression studies revealed that transcripts of a number of plant defense genes, as well as genes involved in SA biosynthesis and signaling, were lower in AtFAAH overexpressors than wild-type plants. Our data suggest that FAAH overexpression alters phytohormone accumulation and signaling which in turn compromises innate immunity to bacterial pathogens.