Differential response of Period 1 expression within the suprachiasmatic nucleus
Differential response of Period 1 expression within the suprachiasmatic nucleus
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DOI：
10.1523/jneurosci.0889-05.2005
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发表时间：
2005-06-08
影响因子：
5.3
通讯作者：
Block, GD
中科院分区：
文献类型：
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作者：
Nakamura, W;Yamazaki, S;Block, GD
下丘脑的视交叉上核(SCN)包含一个生物钟，它对节律的生理和行为施加深刻的控制。时钟由多个自主的细胞起搏器组成，这些起搏器分布在大鼠的SCN中。响应于光调度中的移动，SCN快速改变相位以实现与移动的光调度的适当的相位关系。通过使用转基因大鼠，其中周期1基因的转录节律性是用荧光素酶报告(PER1-LUC)测量的，我们成功地跟踪了SCN不同区域分子节律相位重新调整的时间进程，这些时间进程发生在对光时间表的改变的反应中。我们发现SCN相的不同区域以不同的速率调节，导致SCN区域之间PER1-Luc表达的瞬时内部去同步化。与光调度延迟相比，当光调度提前时，区域之间的这种去同步最为明显和延长。在运动行为中也观察到了类似的相位重置速度的不对称，这表明在SCN内的相移动力学可能是在行为重置中观察到的光时间表提前或延迟的差异的基础。
The suprachiasmatic nuclei (SCNs) of the hypothalamus contain a circadian clock that exerts profound control over rhythmic physiology and behavior. The clock consists of multiple autonomous cellular pacemakers distributed throughout the rat SCN. In response to a shift in the light schedule, the SCN rapidly changes phase to achieve the appropriate phase relationship with the shifted light schedule. Through use of a transgenic rat in which rhythmicity in transcription of the Period 1 gene was measured with a luciferase reporter (Per1-luc), we have been successful in tracking the time course of molecular rhythm phase readjustments in different regions of the SCN that occur in response to a shift in the light schedule. We find that different regions of the SCN phase adjust at different rates, leading to transient internal desynchrony in Per1-luc expression among SCN regions. This desynchrony among regions is most pronounced and prolonged when the light schedule is advanced compared with light schedule delays. A similar asymmetry in the speed of phase resetting is observed with locomotor behavior, suggesting that phase shifting kinetics within the SCN may underlay the differences observed in behavioral resetting to advances or delays in the light schedule.