Sulforaphane controls TPA-induced MMP-9 expression through the NF-κB signaling pathway, but not AP-1, in MCF-7 breast cancer cells.
Sulforaphane controls TPA-induced MMP-9 expression through the NF-κB signaling pathway, but not AP-1, in MCF-7 breast cancer cells.
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Sulforaphane在MCF-7乳腺癌细胞中通过NF-κB信号传导途径（而不是AP-1）控制TPA诱导的MMP-9表达。
DOI：
10.5483/bmbrep.2013.46.4.160
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发表时间：
2013-04
期刊：
影响因子：
3.8
通讯作者：
Kim JS
中科院分区：
文献类型：
--
作者：
Lee YR;Noh EM;Han JH;Kim JM;Hwang BM;Kim BS;Lee SH;Jung SH;Youn HJ;Chung EY;Kim JS
萝卜硫素[1-isothiocyanato-4-（methylsulfinyl）-butane]是一种异硫氰酸酯，存在于一些十字花科蔬菜中，尤其是西兰花。萝卜硫素已显示出对各种癌细胞系的抗癌特性。基质金属蛋白酶-9（MMP-9）降解细胞外基质（ECM），在癌细胞侵袭中起重要作用。在这项研究中，我们研究了萝卜硫素对12-O-十四烷酰基佛波醇-13-乙酸酯（TPA）诱导的MCF-7细胞MMP-9表达和细胞侵袭的影响。莱菔硫烷处理可降低TPA诱导的MMP-9表达和细胞侵袭力。TPA显著增加NF-κB和AP-1的DNA结合活性。莱菔硫烷预处理抑制TPA刺激的NF-κB结合活性，但不抑制AP-1结合活性。此外，我们发现萝卜硫素通过抑制TPA处理的MCF-7细胞中IκB的磷酸化来抑制NF-κB的活化。在这项研究中，我们证明，抑制TPA诱导的MMP-9的表达和细胞侵袭的萝卜硫素是通过抑制NF-κB途径在MCF-7细胞介导的。[BMB报告2013; 46（4）：201-206]
Sulforaphane [1-isothiocyanato-4-(methylsulfinyl)-butane] is an isothiocyanate found in some cruciferous vegetables, especially broccoli. Sulforaphane has been shown to display anti-cancer properties against various cancer cell lines. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix (ECM), plays an important role in cancer cell invasion. In this study, we investigated the effect of sulforaphane on 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced MMP-9 expression and cell invasion in MCF-7 cells. TPA-induced MMP-9 expression and cell invasion were decreased by sulforaphane treatment. TPA substantially increased NF-κB and AP-1 DNA binding activity. Pre-treatment with sulforaphane inhibited TPA-stimulated NF-κB binding activity, but not AP-1 binding activity. In addition, we found that sulforaphane suppressed NF-κB activation, by inhibiting phosphorylation of IκB in TPA-treated MCF-7 cells. In this study, we demonstrated that the inhibition of TPA-induced MMP-9 expression and cell invasion by sulforaphane was mediated by the suppression of the NF-κB pathway in MCF-7 cells. [BMB Reports 2013; 46(4): 201-206]
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