Characterization and bioactivity of nano-submicro octacalcium phosphate/gelatin composite
Characterization and bioactivity of nano-submicro octacalcium phosphate/gelatin composite
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DOI：
10.1016/j.apsusc.2013.05.086
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发表时间：
2013-10-01
影响因子：
6.7
通讯作者：
Suzuki, Osamu
中科院分区：
文献类型：
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作者：
Miura, Kei-ichiro;Anada, Takahisa;Suzuki, Osamu
本研究旨在探讨纳米-亚微米级磷酸八钙（OCP）-分散明胶（Gel）复合材料（nano-submicro OCP/Gel）作为骨替代材料在各种骨缺损中的物理化学和生物活性。将生长良好的合成OCP从100至300 μ m筛分的颗粒机械研磨成尺寸约为500 nm的颗粒。然后，将50重量%的纳米-亚微米OCP与猪皮衍生的酸提取明胶混合。将混合物模塑并冻干，然后进行脱水热交联。X射线衍射（XRD）和傅里叶变换红外（FTIR）光谱表明，OCP的结构即使在机械研磨到纳米-亚微米级水平后仍保持不变，并且在凝胶基质中也有夹杂物存在。将纳米-亚微米OCP/Gel复合材料浸泡在模拟体液中7天，植入大鼠颅骨缺损8周，观察其生物活性。XRD分析表明，纳米-亚微米级的OCP在模拟体液中倾向于转化为低结晶度的羟基磷灰石（HA）。纳米-亚微米OCP/凝胶在体内表现出骨传导性，产生与植入复合材料密切相关的新骨形成。这些结果表明，纳米-亚微米OCP/凝胶复合材料具有与先前报道的其他OCP基材料相似的骨传导性，可用作修复各种骨缺损的骨替代材料。(C)2013 Elsevier B. V.保留所有权利。
The present study was designed to investigate the physicochemical and bioactive properties of a nano-submicro sized octacalcium phosphate (OCP)-dispersed gelatin (Gel) composite (nano-submicro OCP/Gel) used as a bone substitute material in various bone defects. Well-grown, synthesized OCP was mechanically ground from 100 to 300 mu m-sieved granules to particles that were approximately 500 nm in size. Then, 50 wt% of the nano-submicro OCP was mixed with porcine skin-derived acid extracted gelatin. The mixture was molded and lyophilized and then subjected to dehydrothermal crosslinking. X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy showed that the structure of OCP was retained even after mechanical grinding to a nano-submicro scale level as well as inclusion in the Gel matrix. The bioactivity of nano-submicro OCP/Gel was examined by immersing the composite in simulated body fluid (SBF) for 7 days and by implanting it in rat critical-sized calvaria defects for 8 weeks. The nano-submicro OCP tended to convert to low crystalline hydroxyapatite (HA) in SBF as assessed by XRD. The nano-submicro OCP/Gel exhibited osteoconductivity in vivo, yielding new bone formation that was closely associated with the implanted composite. These results suggest that the nano-submicro OCP/Gel composite exhibits similar osteoconductivity as observed in other OCP-based materials previously reported and could be used as a bone substitute material for repairing various defects in bone. (C) 2013 Elsevier B.V. All rights reserved.