Age-associated DNA damage is accelerated in the senescence-accelerated mice
Age-associated DNA damage is accelerated in the senescence-accelerated mice
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DOI：
10.1016/s0047-6374(00)00158-5
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发表时间：
2000-09-01
影响因子：
5.3
通讯作者：
Zahn, RK
中科院分区：
文献类型：
--
作者：
Hosokawa, M;Fujisawa, H;Zahn, RK
我们研究了DNA状态如何与生物体、不同器官和组织中的衰老过程相关，使用了两种近交系小鼠，这两种小鼠具有遗传相关但具有不同的衰老模式。SAMP1小鼠属于易加速衰老的短寿命品系，SAMR1小鼠属于抗加速衰老的长寿命品系。使用碱性过滤洗涤技术，从肺、肠、肝、脑、肌肉和心脏六个器官的组织块中检测DNA损伤，主要是DNA单链断裂。结果表明，在新生儿中，DNA损伤最小，随着年龄的增长，两种菌株的所有器官的DNA损伤都显著增加。尽管DNA损伤与衰老的相关性在不同的6个器官中有所不同，但SAMP1小鼠的所有器官损伤都明显高于SAMR1小鼠。这是另一个DNA损伤与衰老过程密切相关的显著例子，特别是与衰老加速有关。(C) 2000爱思唯尔科学爱尔兰有限公司版权所有。
We investigated how the DNA status correlates with the aging process in organisms, in different organs and in tissues using two inbred strains of mice, which are genetically related but have different senescence patterns. The SAMP1 mice belong to an accelerated senescence-prone and short lived strain, the other, SAMR1 mice are from an accelerated senescence-resistant and long lived strain. Using the alkaline filter elution technique, pieces of tissues from six organs: lung, intestine, liver, brain, muscle, and heart have been examined for DNA damage, mainly DNA single strand breaks. It was shown that in newborns the DNA damage is minimal, and it was increased significantly with calendric age in all organs in both strains. Although the correlation of DNA damage with aging differed in the different six organs, damage was significantly higher in SAMP1 mice than SAMR1 mice at later life in all organs. This is another remarkable example for the strong correlation of DNA damage and aging process, especially with senescence acceleration. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.