Clinical Significance and Prognostic Value of PAX3 Expression in Human Glioma
Clinical Significance and Prognostic Value of PAX3 Expression in Human Glioma
复制标题
DOI：
10.1007/s12031-011-9677-1
复制
发表时间：
2012-05-01
影响因子：
3.1
通讯作者：
Shi, Wei
中科院分区：
文献类型：
--
作者：
Chen, Jian;Xia, Liang;Shi, Wei
PAX 3是一种重要的转录因子，在胚胎发育过程中正常表达，在正常成人组织中不表达。已经在肿瘤如横纹肌肉瘤和黑色素瘤中观察到PAX 3的失调表达。为了评估神经胶质瘤患者中PAX 3表达失调，获得了来自57名神经胶质瘤患者（13名I级、16名II级、14名III级和14名IV级肿瘤）的这些样品和作为对照的从10名接受癫痫手术的患者获得的10个正常脑标本。RT-PCR、Western blot和免疫组化检测PAX 3的表达。使用Kaplan-Meier方法进行生存分析。采用SPSS17.0统计软件分析PAX 3表达与临床病理特征及患者生存期的关系。我们发现PAX 3在高级别胶质瘤组织中的表达高于低级别和正常脑组织，并且随着肿瘤世界卫生组织（WHO）分级的升高而增加（P = 0.001）。PAX 3在胶质瘤中的表达增加与较高的WHO分级（P = 0.021）和较差的疾病特异性生存率（P = 0.001）显著相关。我们的研究结果表明PAX 3可能是胶质瘤细胞进展的内在调节因子，并且可能作为这种令人沮丧的疾病的预后因子。
The paired box 3 (PAX3), a crucial transcription factor, is normally expressed during embryonic development and is absent in normal adult human tissues. Deregulated expression of PAX3 has been observed in tumors like rhabdomyosarcoma and melanomas. To assess deregulated PAX3 expression in patients with gliomas, these samples from 57 glioma patients (13 grade I, 16 grade II, 14 grade III, and 14 grade IV tumors) and 10 normal brain specimens acquired from 10 patients undergoing surgery for epilepsy as control were obtained. PAX3 expression was measured by RT-PCR, Western blot, and immunohistochemistry. Survival analyses were performed using the Kaplan-Meier method. Association between PAX3 expression, clinicopathological characteristics, and patients' survival were analyzed by using SPSS 17.0. We found that the expression of PAX3 was upregulated in high-grade glioma tissues compared with that in low-grade and normal brain tissues, and increased with ascending tumor World Health Organization (WHO) grades (P = 0.001). The increased PAX3 expression in gliomas was significantly associated with higher WHO grade (P = 0.021) and poorer disease-specific survival of patients (P = 0.001). Our results suggested that PAX3 might be an intrinsic regulator of progression in glioma cells and it might serve as a prognostic factor for this dismal disease.