PROTEIN-SYNTHESIS AND AXONAL TRANSPORT IN RETINAL GANGLION-CELLS OF MICE LACKING VISUAL RECEPTORS
PROTEIN-SYNTHESIS AND AXONAL TRANSPORT IN RETINAL GANGLION-CELLS OF MICE LACKING VISUAL RECEPTORS
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DOI：
10.1016/0006-8993(72)90364-2
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发表时间：
1972-01-01
期刊：
影响因子：
2.9
通讯作者：
INGOGLIA, NA
中科院分区：
文献类型：
--
作者：
GRAFSTEI.B;MURRAY, M;INGOGLIA, NA
将具有视觉受体细胞遗传性变性的小鼠的视网膜与具有正常视网膜的近亲品系小鼠的视网膜进行比较。(1)突变体的视网膜神经节细胞减少了20%，其余神经节细胞的大小减少了10-20%。 (2)突变体神经节细胞蛋白中氚化氨基酸的掺入量比正常人少约35%，但标记蛋白以与正常人相同的速度从细胞中消失。 (3)两组动物视神经轴突蛋白转运快速成分的速率相同，最大为190mm/天。 (4) 正常情况下缓慢运输的速度约为每天 1.5 至 3 毫米，而突变体则减少了三分之一。 (5)用脯氨酸和亮氨酸获得的结果基本相同，但表明快速和慢速成分中含有脯氨酸的蛋白质的寿命更长。突变体中视觉受体的缺失很可能导致神经节细胞中电活动水平大大降低，这可能是观察到的变化的基础。另一方面，突变体中快速转运的速率没有变化表明该速率与电活动、细胞大小或蛋白质合成的变化无关。
The retinas of mice with hereditary degeneration of visual receptor cells were compared with those of a closely related strain of mice with normal retinas.(1) The mutants had 20% fewer retinal ganglion cells and the remaining ganglion cells were reduced in size by 10–20%. (2) Tritiated amino acid incorporation into ganglion cell protein was about 35% less in the mutants than in the normals, but the labeled protein disappeared from the cells at the same rate as in the normals. (3) The rate of the fast component of axonal protein transport in the optic nerve was the same in both groups of animals, with a maximum of 190 mm/day. (4) The rate of slow transport, which was between about 1.5 and 3 mm/day in the normals, was reduced by one-third in the mutants. (5) Results obtained with proline and leucine were essentially the same, but indicated a longer lifetime for the proline-containing proteins in both the fast and slow components.The absence of visual receptors in the mutants is likely to result in a greatly reduced level of electrical activity in the ganglion cells, which is probably the basis for the observed changes. On the other hand, the unchanged rate of fast transport in the mutants indicates that this rate is independent of alterations in electrical activity, cell size or protein synthesis.