Antibody in Middle Ear Fluid of Children Originates Predominantly from Sera and Nasopharyngeal Secretions
Antibody in Middle Ear Fluid of Children Originates Predominantly from Sera and Nasopharyngeal Secretions
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DOI：
10.1128/cvi.05443-11
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发表时间：
2012-10-01
影响因子：
--
通讯作者：
Pichichero, Michael E.
中科院分区：
文献类型：
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作者：
Kaur, Ravinder;Kim, Thomas;Pichichero, Michael E.
人中耳在正常粘膜中没有任何免疫活性细胞。我们试图确定儿童中耳中抗体的来源。通过酶联免疫吸附试验测定了急性中耳炎患儿鼻咽、中耳和血清中的总IgG、伊加和分泌型伊加抗体。使用等电聚焦和蛋白质印迹法比较了鼻洗液（NW）和中耳液（MEF）和NW样品中伊加抗体的整个阵列的二维凝胶电泳模式。对137例MEF和NW患儿血清中IgG和伊加抗体进行了比较。MEF中IgG与伊加的比率与NW相比有显著差异（P < 0.008），因为NW中伊加水平较高而IgG水平较低。MEF的IgG/伊加比值与血清相似，与MEF的渗出一致。少量的分泌伊加被检测到MEF，但整个阵列的伊加抗体的MEF和NW的电泳模式几乎是相同的，在每个孩子的评价，因此，伊加在MEF主要来自血清和鼻咽通过回流通过咽鼓管。MEF中的IgG/伊加抗体水平以及MEF和NW中伊加抗体的相同组成确定了MEF中抗体的主要来源为血清渗出液结合儿童鼻咽回流的鼻分泌物。
The human middle ear is devoid of any immunocompetent cells in normal mucosa. We sought to determine the source of antibody present in the middle ear of children. Total IgG, IgA, and secretory IgA antibodies were determined by enzyme-linked immunosorbent assay from the nasopharyngeal, middle ear, and serum samples of children with acute otitis media. The two-dimensional gel electrophoresis pattern of the entire array of IgA antibodies in the nasal wash (NW) and middle ear fluid (MEF) was compared from the MEF and NW samples using isoelectric focusing and Western blotting. The total IgG and IgA antibodies in the MEF and NW samples of 137 children were compared. The ratio of IgG to IgA in the MEF was significantly different (P < 0.008) compared to NW because IgA levels were higher and IgG levels lower in NW. The IgG/IgA ratio of MEF resembled serum consistent with transudation to the MEF. Small amounts of secretory IgA were detected in MEF but the electrophoresis patterns of the entire array of IgA antibodies in the MEF and NW were virtually identical in each child evaluated; thus, IgA in MEF derived predominantly from serum and the nasopharynx by reflux via the Eustachian tube. The IgG/IgA antibody levels in the MEF and the same composition of IgA antibody in the MEF and NW identifies the predominant source of antibody in the MEF as a transudate of serum combined with nasal secretions refluxed from the nasopharynx in children.