EFFECT OF HORSERADISH PEROXIDASE ON THE BIOSYNTHESIS OF ARTEMISININ IN ARTEMISIA ANNUA IN VITRO
EFFECT OF HORSERADISH PEROXIDASE ON THE BIOSYNTHESIS OF ARTEMISININ IN ARTEMISIA ANNUA IN VITRO
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辣根过氧化物酶对青蒿体外青蒿素生物合成的影响
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
Ye Hechun
中科院分区：
文献类型：
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作者：
Ye Hechun
本文研究了辣根过氧化物酶对青蒿素生物合成的影响。使用两种缓冲液系统制备无细胞浸提液。结果表明，通过磷酸盐缓冲体系将过氧化物酶包埋在无细胞提取液中，青蒿素的产量可提高1倍左右，外源青蒿酸的加入对青蒿素的产量影响不大。本研究表明，磷酸盐缓冲体系比Tris缓冲体系更有利于过氧化物酶的活性。在Tris缓冲体系的无细胞提取液中，添加辣根过氧化物酶并不能促进青蒿素的生物合成。图4，参考文献12
In this paper,the effect of horseradish peroxidase on the biosynthesis of artemisinin was studied. Two buffer systems were used to prepare cell free extracts. The results showed that the level of artemisinin was elevated to about 1-fold when the peroxidase was included in cell free extracts by phosphate buffer system,and the added exogenous artemisinic acid could not greatly affect the production of artemisinin. This study indicated that phosphate buffer system was better for peroxidase activities than Tris buffer system. In the cell free extracts by Tris buffer system,addition of horseradish peroxidase did not promote the biosynthesis of artemisinin. Fig 4,Ref 12