Heterogeneity in surface sensing suggests a division of labor in Pseudomonas aeruginosa populations
Heterogeneity in surface sensing suggests a division of labor in Pseudomonas aeruginosa populations
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表面传感的异质性表明铜绿假单胞菌种群存在分工
DOI：
10.7554/elife.45084
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发表时间：
2019-06-10
期刊：
影响因子：
7.7
通讯作者：
Parsek, Matthew R.
中科院分区：
文献类型：
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作者：
Armbruster, Catherine R.;Lee, Calvin K.;Parsek, Matthew R.
第二信使信号分子环二鸟苷一磷酸(c-di-GMP)在许多细菌物种中驱动浮游和生物膜生长之间的过渡。铜绿假单胞菌有两个表面感应系统，可在表面附着时产生c-di-GMP。目前该领域的想法是，一旦细胞附着在表面，它们就会通过产生c-di-GMP来统一做出反应。在这里，我们描述了WSP系统如何在表面传感中产生异质性，从而产生两个生理上不同的细胞亚群。其中一个亚群c-di-GMP升高，并产生生物膜基质，作为初始微克隆的创建者。另一个亚群的c-di-GMP含量较低，并从事表面运动，允许对表面进行探索。我们还表明，这种异质性与后代细胞的表面行为密切相关。综上所述，我们的结果表明，在表面附着后，铜绿假单胞菌参与了一种跨越世代的劳动分工，加速了早期生物膜的形成和表面探索。
The second messenger signaling molecule cyclic diguanylate monophosphate (c-di-GMP) drives the transition between planktonic and biofilm growth in many bacterial species. Pseudomonas aeruginosa has two surface sensing systems that produce c-di-GMP in response to surface adherence. Current thinking in the field is that once cells attach to a surface, they uniformly respond by producing c-di-GMP. Here, we describe how the Wsp system generates heterogeneity in surface sensing, resulting in two physiologically distinct subpopulations of cells. One subpopulation has elevated c-di-GMP and produces biofilm matrix, serving as the founders of initial microcolonies. The other subpopulation has low c-di-GMP and engages in surface motility, allowing for exploration of the surface. We also show that this heterogeneity strongly correlates to surface behavior for descendent cells. Together, our results suggest that after surface attachment, P. aeruginosa engages in a division of labor that persists across generations, accelerating early biofilm formation and surface exploration.