Characterization of a monoclonal antibody against concanavalin A binding antigen of Aspergillus fumigatus.
Characterization of a monoclonal antibody against concanavalin A binding antigen of Aspergillus fumigatus.
复制标题
抗烟曲霉伴刀豆球蛋白 A 结合抗原的单克隆抗体的表征。
作者：
Kurup,VP;Elms,N;Fink,JN
A monoclonal antibody was produced against a Concanavalin A (Con A) binding major epitope ofAspergillus fumigatususing a novel method of immunization. The antigen was purified using monoclonal antibody affinity chromatography reacted with specific antibodies present in human sera. Both allergic bronchopulmonary aspergillosis and aspergilloma showed high levels of antibody, against this purified antigen, when compared to normal controls. Similar results were obtained when the monoclonal antibody was used in a capture antigen assay. The antibody reacted with severalA. fumigatusextracts in rocket electrophoresis demonstrating a single precipitin arc, which disappeared when Con A intermediate gel was used. This monoclonal antibody demonstated reactivity only with cytoplasmic components of hyphae and spores ofA. fumigatus, when a colloidal gold was used as a probe in immunoelectron microscopy.
