Two roles for the leucine-responsive regulatory protein in expression of the alanine catabolic operon (dadAB) in Klebsiella aerogenes.
Two roles for the leucine-responsive regulatory protein in expression of the alanine catabolic operon (dadAB) in Klebsiella aerogenes.
复制标题
亮氨酸反应性调节蛋白在产气克雷伯菌丙氨酸分解代谢操纵子 (dadAB) 表达中的两个作用。
DOI：
10.1128/jb.181.3.1054-1058.1999
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发表时间：
1999
影响因子：
3.2
通讯作者：
Bender,RA
中科院分区：
文献类型：
--
作者：
Janes,BK;Bender,RA
Thelrpgene, which codes for the leucine-responsive regulatory protein (Lrp), was cloned fromKlebsiella aerogenesW70. The DNA sequence was determined, and the clone was used to create a disruption of thelrpgene. The lack of functional Lrp led to an increased expression of the alanine catabolic operon (dad) in the absence of the inducerl-alanine but also to a decreased expression of the operon in the presence ofl-alanine. Thus, Lrp is both a repressor and activator ofdadexpression. Lrp is also necessary for glutamate synthase formation but not for the formation of two other enzymes controlled by the nitrogen regulatory (Ntr) system, glutamate dehydrogenase and histidase.
