Structure of the chromosomal gene for granulocyte‐macrophage colony stimulating factor: comparison of the mouse and human genes.
Structure of the chromosomal gene for granulocyte‐macrophage colony stimulating factor: comparison of the mouse and human genes.
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粒细胞巨噬细胞集落刺激因子染色体基因的结构：小鼠和人类基因的比较。
DOI：
10.1002/j.1460-2075.1985.tb03971.x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
K. Arai
中科院分区：
文献类型：
--
作者：
S. Miyatake;T. Otsuka;T. Yokota;F. Lee;K. Arai
在COS - 7细胞中表达粒细胞-巨噬细胞集落刺激因子（GM - CSF）活性的cDNA克隆已从从豆豆蛋白A激活的小鼠辅助性T细胞克隆的mRNA制备的pcD文库中分离出来。基于与小鼠GM - CSF cDNA序列的同源性，分离得到小鼠GM - CSF基因。根据与人GM - CSF cDNA序列的同源性，分离出人GM - CSF基因。基因及其侧翼区域的核苷酸序列显示，小鼠和人类GM - CSF基因都由3个内含子和4个外显子组成。小鼠和人GM - CSF基因的组织结构高度同源，在编码区和非编码区都有很强的序列同源性。在转录起始位点上游20‐25bp处发现了一个‘ TATA ’样序列。在5 '侧翼区域，有一个高度同源的区域，在假定的TATA盒的上游延伸330bp。该序列可能在GM - CSF基因的表达调控中发挥作用。这些结构与不同的淋巴因子基因及其调控区域的结构进行了比较。
A cDNA clone that expresses granulocyte‐macrophage colony stimulating factor (GM‐CSF) activity in COS‐7 cells has been isolated from a pcD library prepared from mRNA derived from concanavalin A‐activated mouse helper T cell clones. Based on homology with the mouse GM‐CSF cDNA sequence, the mouse GM‐CSF gene was isolated. The human GM‐CSF gene was also isolated based on homology with the human GM‐CSF cDNA sequence. The nucleotide sequences determined for the genes and their flanking regions revealed that both the mouse and human GM‐CSF genes are composed of three introns and four exons. The organization of the mouse and human GM‐CSF genes are highly homologous and strong sequence homology between the two genes is found both in the coding and non‐coding regions. A ‘TATA’‐like sequence was found 20‐25 bp upstream from the transcription initiation site. In the 5′‐flanking region, there is a highly homologous region extending 330 bp upstream of the putative TATA box. This sequence may play a role in regulation of expression of the GM‐CSF gene. These structures are compared with those of different lymphokine genes and their regulatory regions.