DOG AND HUMAN ACID BETA-D-GALACTOSIDASES ARE STRUCTURALLY SIMILAR
DOG AND HUMAN ACID BETA-D-GALACTOSIDASES ARE STRUCTURALLY SIMILAR
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DOI：
10.1042/bj2130473
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发表时间：
1983-01-01
影响因子：
4.1
通讯作者：
OBRIEN, JS
中科院分区：
文献类型：
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作者：
HUBERT, JJ;OBRIEN, JS
狗肝酸β的纯化描述了半乳糖苷酶。在十二烷基硫酸钠存在下，犬酶在聚丙烯酰胺凝胶电泳上迁移为单一主带，分子量为60，000。针对纯化的人肝酸β-半乳糖苷酶与β-半乳糖苷酶从狗的肝脏，但不与那些从猫的肝脏或大肠杆菌。狗和人酸β的胰蛋白酶肽图谱-半乳糖苷酶的结果表明，观察到的24种肽中有21种是同源的;胰凝乳蛋白酶肽作图后获得了类似的结果。狗和人的酸β-半乳糖苷酶在结构上相似，并且犬GM 1神经节苷脂沉积症（酸性β-半乳糖苷脂酶）在结构上相似。半乳糖苷酶缺乏症）是人类相同疾病的极好模型。
The purification of dog liver acid .beta.-galactosidase is described. The dog enzyme migrated as a single major band on polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulfate, with a MW of 60,000. Antiserum raised against purified human liver acid .beta.-galactosidase cross-reacted with .beta.-galactosidase from the dog liver, but not with those from cat liver or Escherichia coli. Tryptic peptide maps of the dog and human acid .beta.-galactosidases indicate that 21 of the 24 peptides observed were homologous; a similar result was obtained after chymotryptic peptide mapping. Dog and human acid .beta.-galactosidases are structurally similar, and canine GM1 gangliosidosis (acid .beta.-galactosidase deficiency) is an excellent model for the same disease in man.