A straightforward route to the synthesis of a surface-enhanced Raman scattering probe for targeting transferrin receptor-overexpressed cells
A straightforward route to the synthesis of a surface-enhanced Raman scattering probe for targeting transferrin receptor-overexpressed cells
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DOI：
10.1088/0957-4484/21/34/345101
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发表时间：
2010-08
期刊：
影响因子：
3.5
通讯作者：
Jing Yang;Zhuyuan Wang;Xuebin Tan;Jin Li;Chunyuan Song;Ruohu Zhang;Yiping Cui
中科院分区：
文献类型：
--
作者：
Jing Yang;Zhuyuan Wang;Xuebin Tan;Jin Li;Chunyuan Song;Ruohu Zhang;Yiping Cui
A tumor cell targeting surface-enhanced Raman scattering (SERS) probe has been successfully synthesized by using p-mercaptobenzoic acid (pMBA) as both the SERS reporter and the conjugation agent for attaching transferrin molecules, which shows experimentally the targeting ability for transferrin receptor-overexpressed HeLa cells and exhibits strong SERS signals when being incubated inside cells. To prove that the uptake of such a SERS probe is through a Tf-receptor-mediated endocytosis process, two control experiments: (1) HeLa cells being incubated with the probe at 4 °C and (2) HeLa cells being pre-blocked with free transferrin at 37 °C, were employed. The difference of SERS intensity between the transferrin-overexpressed HeLa cells and transferrin-pre-blocked HeLa cells indicates that the probe has the potential to selectively target tumor cells.
