A novel strategy for enhancing inhibitor tolerance of gram-positive bacteria through overexpression of the peptidoglycan synthesis genes murG and mraY
A novel strategy for enhancing inhibitor tolerance of gram-positive bacteria through overexpression of the peptidoglycan synthesis genes murG and mraY
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DOI：
10.1016/j.indcrop.2022.116112
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发表时间：
2023-02
影响因子：
5.9
通讯作者：
Yejuan Qiu;Zhongyang Qiu;Ai-yong He;Jiaxing Xu;Xingcui Yang;Hanwen Zhang;Yuxiang Yang;Wenyi Hou;Xiangqian Li;Jianlong He
中科院分区：
文献类型：
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作者：
Yejuan Qiu;Zhongyang Qiu;Ai-yong He;Jiaxing Xu;Xingcui Yang;Hanwen Zhang;Yuxiang Yang;Wenyi Hou;Xiangqian Li;Jianlong He
木质纤维素的前处理会产生抑制物，如酚醛、呋喃衍生物和弱酸。在本研究中，mR-GandmraY的过表达和共表达增强了对这些抑制剂的耐受性。乳酸链球菌(PZY36e-mray)在添加0.8 mg/L香兰素、6 mg/L对羟基苯甲醛、1.5 mg/L丁香醛、1.0%(v/v)糠醛、1.0%(v/v)呋喃甲醛和1.0%(v/v)羟甲基纤维素时，24 h的产酸率分别为380%、22%、28%、22%和35%。OFP的存活率。以1.5 mg/L香兰素、10 mg/L 4-羟基苯甲醛、2.5 mg/L丁香醛、1.8%(v/v)呋喃甲醛和1.2%(v/v)氢氧化氢孵育2 h，乳酸杆菌(pZY36e-mray)的产酶活力分别提高6.2倍、9.9倍、2.4倍、6.5倍和6.0倍。此外，重组子P。耐酸菌株能耐受玉米芯的预处理液，从而促进乳酸的产生。小鼠GandmraY的过表达诱导乳酸乳球菌的高耐受性。对乳酸乳球菌(pZY36e-mray)细胞壁的透射电子显微镜观察表明，在抑制剂存在的情况下，细胞壁的损伤较小。总体而言，这一策略可以用于提高其他革兰氏阳性细菌对各种抑制剂的耐受性。
Pretreatment of lignocellulose can generate inhibitors, such as phenolic aldehyde, furan derivatives, and weak acids. In this study, the overexpression and co-overexpression ofmurGandmraYenhanced the tolerance against these inhibitors. The lactic acid production rates by Pediococcus acidilactici (pZY36e-mraY) in the presence of 0.8 g/L vanillin, 6 g/L 4-hydroxybenzaldehyde, 1.5 g/L syringaldehyde, 1.0% (v/v) furfural, and 1.0% (v/v) HMF at 24 h were 380%, 22%, 28%, 22%, and 35%, respectively, which were higher than that of the control strain. The survival rates ofP. acidilactici(pZY36e-mraY) incubated for 2 h with 1.5 g/L vanillin, 10 g/L 4-hydroxybenzaldehyde, 2.5 g/L syringaldehyde, 1.8% (v/v) furfural, and 1.2% (v/v) HMF were significantly increased by 6.2, 9.9, 2.4, 6.5, and 6.0 folds, respectively. Additionally, the recombinantP. acidilacticistrains could tolerate the presence of pretreated liquor from corn cob, thus promoting lactic acid production. Overexpression ofmurGandmraYinduced high tolerance in Lactococcus lactis. The TEM images showed that the cell wall of Lactococcus lactis (pZY36e-mraY) was less damaged in the presence of inhibitors. Overall, this strategy can be employed to improve the tolerance of other gram-positive bacteria against various inhibitors.