Development of an in-vitro method for simulation of oxygen transport in the microcirculation.
Development of an in-vitro method for simulation of oxygen transport in the microcirculation.
复制标题
开发模拟微循环中氧气输送的体外方法。
DOI：
10.1007/978-1-4684-3291-6_94
复制
发表时间：
1985
影响因子：
--
通讯作者：
Hellums,JD
中科院分区：
文献类型：
--
作者：
Boland,EJ;Olson,JS;Hellums,JD
Measurement of oxygen transport rates in the microcirculation has been the subject of numerousin vivoinvestigations and considerable progress has been made. However, there are experimental difficulties in exact determination of several important parameters including the capillary wall boundary conditions and the capillary dimensions. These difficulties have constituted the incentive for our continuing development of anin vitrosystem in which the capillary dimensions are determined precisely by light and electron microscopy; the flow rate is carefully regulated; the inlet concentration of red cells or hemoglobin is controlled independently; the fractional saturation of hemoglobin is measured spectrophotometrically; and the boundary conditions in the silicone rubber capillary bed can be computed by established mathematical techniques. Accurate measurement of these variables should prove to be useful in testing and validating the several mathematical models which have been used in simulation of microcirculatory oxygen transport. A preliminary report on the project has described the basic features of the method (Boland, et al, 1984).
