Dietary Enteromorpha Polysaccharides Supplementation Improves Breast Muscle Yield and Is Associated With Modification of mRNA Transcriptome in Broiler Chickens.
Dietary Enteromorpha Polysaccharides Supplementation Improves Breast Muscle Yield and Is Associated With Modification of mRNA Transcriptome in Broiler Chickens.
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DOI：
10.3389/fvets.2021.663988
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发表时间：
2021
影响因子：
3.2
通讯作者：
Liu WC
中科院分区：
文献类型：
--
作者：
Zhao Y;Balasubramanian B;Guo Y;Qiu SJ;Jha R;Liu WC
本研究采用RNA测序技术（RNA-Seq）分析了浒苔多糖（Enteromorpha polysaccharides，EP）对肉仔鸡胴体性状的影响及其可能的分子机制。共396只1日龄雄性肉仔鸡（Arbor Acres）被随机分配到含有0（CON）、1000（EP_1000）、2500（EP_2500）、4000（EP_4000）、5500（EP_5500）、和7000（EP_7000）mg/kg水平，用于35天饲养试验（6个重复/处理）。在饲养试验结束时，从每个处理中随机选择六只鸟（每个重复笼中一只鸟）并屠宰用于胴体性状分析。结果表明，日粮中添加EP_7000能显著提高胸肌产量（p < 0.05）。随后，随机选择来自CON和EP_7000组的六个胸肌样品（每组三个样品）用于RNA-Seq分析。基于RNA-Seq结果，鉴定了总共154个差异表达基因（DEG）（p < 0.05）。在DEG中，112个基因被显著上调，而42个基因被显著下调。Gene Ontology富集分析显示，DEG主要富集在免疫相关信号通路、大分子生物合成、DNA模板化、RNA生物合成和代谢过程中（p < 0.05）。京都基因百科全书和基因组通路分析显示，DEG富含与病毒感染性疾病相关的信号通路和细胞粘附分子（p < 0.05）。因此，日粮中添加EP_7000可提高肉鸡胸肌产量，其机制可能与提高肉鸡免疫力和细胞分化能力，从而促进肌肉生长有关。这些结果有助于了解日粮中添加EP提高肉鸡胸肌产量的分子机制。
The present study evaluated the effects of dietary supplementation of Enteromorpha polysaccharides (EP) on carcass traits of broilers and potential molecular mechanisms associated with it. This study used RNA-Sequencing (RNA-Seq) to detect modification in mRNA transcriptome and the cognate biological pathways affecting the carcass traits. A total of 396 one-day-old male broilers (Arbor Acres) were randomly assigned to one of six dietary treatments containing EP at 0 (CON), 1000 (EP_1000), 2500 (EP_2500), 4000 (EP_4000), 5500 (EP_5500), and 7000 (EP_7000) mg/kg levels for a 35-d feeding trial with 6 replicates/treatment. At the end of the feeding trial, six birds (one bird from each replicate cage) were randomly selected from each treatment and slaughtered for carcass traits analysis. The results showed that the dietary supplementation of EP_7000 improved the breast muscle yield (p < 0.05). Subsequently, six breast muscle samples from CON and EP_7000 groups (three samples from each group) were randomly selected for RNA-Seq analysis. Based on the RNA-Seq results, a total of 154 differentially expressed genes (DEGs) were identified (p < 0.05). Among the DEGs, 112 genes were significantly upregulated, whereas 42 genes were significantly down-regulated by EP_7000 supplementation. Gene Ontology enrichment analysis showed that the DEGs were mainly enriched in immune-related signaling pathways, macromolecule biosynthetic, DNA-templated, RNA biosynthetic, and metabolic process (p < 0.05). Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the DEGs were enriched in signaling pathways related to viral infectious diseases and cell adhesion molecules (p < 0.05). In conclusion, dietary inclusion of EP_7000 improves the breast muscle yield, which may be involved in improving the immunity and the cell differentiation of broilers, thus promoting the muscle growth of broilers. These findings could help understand the molecular mechanisms that enhance breast muscle yield by dietary supplementation of EP in broilers.
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影响因子：
4.4
作者：
Bai H;Bao Q;Zhang Y;Song Q;Liu B;Zhong L;Zhang X;Wang Z;Jiang Y;Xu Q;Chang G;Chen G
通讯作者：
Chen G
影响因子：
0.5
作者：
Jiao, Yang;Jha, Rajesh;Kim, In Ho
通讯作者：
Kim, In Ho
DOI：
10.1016/j.ijbiomac.2018.12.207
发表时间：
2019-04-01
影响因子：
8.2
作者：
Liu, Tianlong;Zhang, Mingjie;Liu, Xiaolei
通讯作者：
Liu, Xiaolei
DOI：
10.1016/j.biocel.2016.10.018
发表时间：
2016-12-01
影响因子：
4
作者：
Kim, Jina;Lee, Yu-Jin;Kwon, Byoung-Mog
通讯作者：
Kwon, Byoung-Mog
DOI：
10.3390/ani10101863
发表时间：
2020-10-13
期刊：
Animals : an open access journal from MDPI
影响因子：
--
作者：
Jha R;Das R;Oak S;Mishra P
通讯作者：
Mishra P