Expression of vascular endothelial growth factor protein in human renal cell carcinoma
Expression of vascular endothelial growth factor protein in human renal cell carcinoma
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DOI：
10.1111/j.1464-410x.2004.04605.x
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发表时间：
2004-02-01
影响因子：
4.5
通讯作者：
Ljungberg, B
中科院分区：
文献类型：
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作者：
Jacobsen, J;Grankvist, K;Ljungberg, B
目的探讨血管内皮生长因子在肿瘤治疗中的作用（VEGF，肾细胞癌（RCC）中最重要的血管生成因子之一），通过分析许多RCC的免疫组织化学（IHC）VEGF染色的表达与临床病理结果和生存率相关。方法对229例患者的肿瘤样品进行了检测，并在71例普通组织切片（TS）中进行了验证。通过估计体积密度和染色强度，以三级量表定量IHC VEGF表达。在大多数RCC中，细胞质和细胞膜中存在VEGF染色。在细胞膜中，VEGF的表达随着储存时间的延长而下降。通过TMA和TS分析的IHC VEGF表达给出了相应的结果。VEGF在常规型、乳头型和嫌色型肾癌中的表达无差异。VEGF的表达与肿瘤大小、分期有关。在单因素分析中，VEGF表达与生存率相关，尤其是在传统RCC中;在多因素分析中，这一预后信息丢失。VEGF的表达强度与肾癌组织中VEGF的表达有关，与肾癌的临床病理因素无关。VEGF在不同类型肾细胞癌中的表达差异无统计学意义。VEGF表达与肿瘤分期及预后的相关性表明VEGF在肾癌的生长和进展中具有重要意义。
OBJECTIVETo evaluate the effect of vascular endothelial growth factor (VEGF, one of the most important angiogenetic factors) in renal cell carcinoma (RCC) by analysing many RCCs for the expression of immunohistochemical (IHC) VEGF‐staining related to clinicopathological findings and survival.PATIENTS AND METHODSVEGF immunostaining was examined with the tissue microarray (TMA) method on tumour samples from 229 patients and validated in 71 by ordinary tissue sections (TS). IHC VEGF expression was quantified by estimating the volume density and staining intensity on a three‐grade scale.RESULTSIn most RCCs there was VEGF staining in the cell cytoplasm and membrane. In cell membranes the VEGF expression declined with storage time. IHC VEGF expression analysed by TMA and TS gave corresponding results. There was no difference in VEGF expression among conventional, papillary and chromophobe RCCs. There were significant correlations between VEGF expression and tumour size and stage. In univariate analysis VEGF expression correlated with survival, especially in conventional RCCs; this prognostic information was lost in multivariate analysis. The VEGF staining intensity correlated only with VEGF expression but not with any clinicopathological factors.CONCLUSIONSVEGF protein was present in most RCC cells. There was no difference in VEGF expression among the different RCC types. The correlation between VEGF expression and tumour stage and with prognosis indicates the significance of VEGF within tumour growth and progression in RCC.