Core-APOBEC3C chimerical protein inhibits hepatitis B virus replication
Core-APOBEC3C chimerical protein inhibits hepatitis B virus replication
复制标题
Core-APOBEC3C嵌合蛋白抑制乙型肝炎病毒复制
DOI：
10.1093/jb/mvr086
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发表时间：
2011-10-01
影响因子：
2.7
通讯作者：
Sun, Dianxing
中科院分区：
文献类型：
--
作者：
Li, Dong;Liu, Jinxia;Sun, Dianxing
我们测试了衣壳靶向病毒灭活方法作为抗HBV策略。用HBV表达质粒和编码Core-A3 C或Core-humanized renilla GFP（hrGFP）的融合蛋白的质粒共转染HepG 2细胞。Core-A3 C对HBV DNA水平有显著影响。在表达Core-A3 C的HepG 2细胞中，G-to-A突变的数量急剧增加，而其他核苷酸取代则很少。此外，核心A3 C实质上抑制HBV的生产细胞内和培养上清液。这些结果表明，Core-A3 C可能是一种新的抗HBV感染的候选药物。
We tested the capsid targeted viral inactivation method as an anti-HBV strategy. HepG2 cells were cotransfected with HBV expression plasmid and the plasmid encoding fusion protein of either Core-A3C or Core-humanized renilla GFP (hrGFP). Core-A3C had substantial effect on HBV DNA levels. In the HepG2 cells expressing Core-A3C, the number of G-to-A mutations increased dramatically, whereas other nucleotide substitutions were rare. In addition, Core-A3C substantially inhibited HBV production intracellularly and in culture supernatant. These results suggest that Core-A3C may be a candidate as a novel antiviral agent against human HBV infection.