THE SIGNALING MOLECULE BMP4 MEDIATES APOPTOSIS IN THE RHOMBENCEPHALIC NEURAL CREST
THE SIGNALING MOLECULE BMP4 MEDIATES APOPTOSIS IN THE RHOMBENCEPHALIC NEURAL CREST
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DOI：
10.1038/372684a0
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发表时间：
1994-12-15
期刊：
影响因子：
64.8
通讯作者：
LUMSDEN, A
中科院分区：
文献类型：
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作者：
GRAHAM, A;FRANCISWEST, P;LUMSDEN, A
头部鳃区的骨骼结构和肌肉模式受到神经嵴的深刻影响，神经嵴的细胞出现在鸡后脑的离散节段水平：特别是菱形节（r）1 + 2、r4和r6，而r3和r5是嵴缺失的（2）。我们已经证明，偶数菱形和r3/r5之间的相互作用会影响神经嵴的消耗，导致神经嵴离散迁移流的雕刻（3）。这种机制通过增加msx 2的表达和诱导r3和r5背侧细胞的凋亡而起作用（参考文献3）（图1A）。在这里，我们表明，信号分子Bmp 4在R3和R5中表达，并依赖于相邻的菱形。将重组BMP 4蛋白添加到r3或r5的外植体培养物中，r3或r5在与其相邻的菱形节分离时产生神经嵴，上调msx 2并恢复神经嵴群体中的细胞凋亡。
THE pattern of skeletal structures and muscles in the branchial region of the head is profoundly influenced by the neural crest whose cells arise at discrete segmental levels of the chick hindbrain: specifically, rhombomeres (r)1 + 2, r4 and r6, whereas r3 and r5 are crest-depleted(2). We have demonstrated that an interaction between even-numbered rhombomeres and r3/r5 effects this depletion of neural crest, resulting in the sculpting of discrete migratory streams of neural crest(3). This mechanism acts through increased expression of msx2 and the induction of apoptosis in dorsal cells of r3 and r5 (ref. 3) (Fig. 1A). Here we demonstrate that the signalling molecule Bmp4 is expressed in r3 and r5 and is dependent on the neighbouring rhombomeres. Addition of recombinant BMP4 protein to explant cultures of r3 or r5, which produce neural crest when isolated from their neighbouring rhombomeres, upregulates msx2 and reinstates apoptosis in the neural crest population.