Exclusion of plasma proteins in interstitium of tissues from the dog hind paw.
Exclusion of plasma proteins in interstitium of tissues from the dog hind paw.
复制标题
排除狗后爪组织间质中的血浆蛋白。
DOI：
10.1152/ajpheart.1980.239.4.h532
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Renkin,EM
中科院分区：
文献类型：
--
作者：
Bell,DR;Watson,PD;Renkin,EM
用放射性示踪法研究了麻醉犬后爪组织中部分血管外蔗糖间隙对血浆白蛋白和纤维蛋白原的排斥作用。在注射标记蛋白30小时后，测量血浆、结前窝淋巴和后爪皮肤、肌腱、肌肉和脚趾垫样本中的示踪剂浓度。在一些实验中，示踪蔗糖的血浆水平在结束采样前保持2小时不变。在所有实验中，在终止前15分钟给予血管容量标记物。组织样本中血管外蛋白示踪剂的数量与其在同一爪子淋巴中的浓度之间存在线性相关，如果它们的实际间质浓度与其淋巴浓度密切相关，这是可以预期的。对于白蛋白，根据淋巴浓度计算的血管外分布体积小于除脚趾垫外的所有组织的蔗糖空间。对于纤维蛋白原，除皮肤外，其余组织的血管外分布体积均小于白蛋白和蔗糖。排斥模式和程度的不同可能反映了间质凝胶相组成和结构的不同。
Exclusion of plasma albumin and fibrinogen from portions of the extravascular sucrose space in tissues of the hind paw of anesthetized dogs was studied by use of radioactive traces. Thirty hours after a bolus injection of the labeled proteins, tracer concentrations were measured in plasma, prenodal popliteal lymph, and samples of hind paw skin, tendon, muscle, and toe pad. In a few experiments, constant plasma levels of tracer sucrose were maintained for 2 h before terminal sampling. In all experiments a vascular volume marker was given 15 min before termination. A linear correlation was found between the amounts of extravascular protein tracers in the tissue samples and their concentrations in lymph from the same paw, as would be expected if their actual interstitial concentrations were closely related to their lymph concentrations. For albumin, the extravascular distribution volume calculated at lymph concentration was less than the sucrose space for all tissues except toe pad. For fibrinogen, the extravascular distribution volume was less than albumin and sucrose for all tissues except skin. Differences in pattern and degree of exclusion may reflect the differences in interstitial gel-phase composition and structure.