EXPRESSION OF TYPE-I AND TYPE-III COLLAGEN DURING MORPHOGENESIS OF FETAL-RAT TESTIS AND OVARY
EXPRESSION OF TYPE-I AND TYPE-III COLLAGEN DURING MORPHOGENESIS OF FETAL-RAT TESTIS AND OVARY
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DOI：
10.1002/ar.1092190115
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发表时间：
1987-09-01
影响因子：
--
通讯作者：
PARANKO, J
中科院分区：
文献类型：
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作者：
PARANKO, J
用免疫细胞化学和超微结构方法研究了从未分化期（第12天）至出生的胎鼠睾丸和卵巢中I型和III型间质胶原的分布。结果表明，间充质的分化、组织和丰度与睾丸索和卵巢索的分化之间存在相关性。III型胶原蛋白已经存在于未分化的性腺间质中，并且它在组织性腺索周围的早期阶段出现。I型胶原后来出现在与III型胶原类似的间充质分布中。在性腺开始形态发生时，上皮下基底膜的碎裂被认为表明表面上皮参与了性腺索的形成。III型胶原蛋白首先在发育中的睾丸索表面表达，然后在卵巢索周围表达，这表明间充质前肌样细胞积极参与了雄性索的形成。在卵巢索的基底膜中发现局灶性不连续，卵巢索部分被分支且相对稀疏的间充质彼此分开。细胞骨架和细胞外基质之间的复杂联系在索状支持细胞和颗粒细胞以及相邻的间充质细胞中都得到了说明。广泛的卵巢髓索的生殖细胞-颗粒细胞协会周围的间充质和基底膜的消耗可能是其随后变性的原因。
The distribution of interstitial collagen type I and III was studied immunocytochemically and ultrastructurally in the fetal rat testis and ovary from the undifferentiated stage (day 12) until birth. The results suggest that there is a correlation between the differentiation, organization, and abundance of the mesenchyme and the differentiation of the testicular vs. ovarian cords. Type III collagen was already present in the undifferentiated gonadal mesenchyme, and it appeared at an early stage around the organizing gonadal cords. Type I collagen appeared later in a similar mesenchymal distribution as type III collagen. Fragmentation of the subepithelial basement membrane in the gonads starting morphogenesis was considered to indicate that the surface epithelium participates in the gonadal cord formation. The expression of type III collagen at first on the surface of the developing testicular cords and later around the ovarian cords suggests that the mesenchymal premyoid cells are actively involved in the male cord formation. Focal discontinuities were found in the basement membrane of the ovarian cords, which in part were separated from each other by a ramified and relatively sparse mesenchyme. A complex linkage between the cytoskeleton and the extracellular matrix is illustrated both in the cord forming Sertoli and granulosa cells, and the adjacent mesenchymal cells. Depletion of the mesenchyme and the basement membrane around the germ cell-granulosa cell associations of the wide ovarian medullary cords may be causal for their subsequent degeneration.