Lower Senescence of Adipose-Derived Stem Cells than Donor-Matched Bone Marrow Stem Cells for Surgical Ventricular Restoration.
Lower Senescence of Adipose-Derived Stem Cells than Donor-Matched Bone Marrow Stem Cells for Surgical Ventricular Restoration.
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DOI：
10.1089/scd.2017.0271
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发表时间：
2018-05
影响因子：
4
通讯作者：
Hua Wu;Jian-Zhong Li;Baodong Xie;Hai Tian;Shao-Hong Fang;Shu-lin Jiang;K. Kang
中科院分区：
文献类型：
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作者：
Hua Wu;Jian-Zhong Li;Baodong Xie;Hai Tian;Shao-Hong Fang;Shu-lin Jiang;K. Kang
外科心室重建术（SVR）能在一定程度上恢复左室动脉瘤患者的心功能。然而，在这种治疗中使用的贴片有一些局限性，如僵硬和钙化。工程心脏组织（EHTs）是一种很有前途的修复心脏损伤的生物材料。然而，为eht选择最佳候选细胞一直存在争议。衰老是老年患者种子细胞的主要考虑因素。因此，本研究旨在评估衰老相关因子（如SA-β-Gal、细胞周期蛋白依赖性激酶抑制剂2A （P16）、细胞周期蛋白依赖性激酶抑制剂1 (P21)）在脂肪源性干细胞（ADSCs）和骨髓干细胞（BMSCs）中的增殖、抗凋亡潜力和表达。此外，在体内评估SVR后ADSCs和BMSCs的心功能、细胞存活和血管生成。体外实验结果显示，老龄ADSCs （OAs）生长较快；SA-β-Gal、P16、P21表达水平较低；并且具有比老BMSCs （OBs）更明显的抗凋亡活性。体内实验结果显示，贴片植入28天后，接受OAs贴片的动物心脏功能恢复优于接受OBs贴片的动物。同时，老的ADSCs在细胞存活和血管生成方面具有更大的潜力。这些结果表明，在老年患者自体细胞移植方面，ADSCs可能优于BMSCs。
Surgical ventricular reconstruction (SVR) can restore cardiac function for left ventricular aneurysm to some extent. However, the patches used in this treatment have some limitations such as stiffness and calcification. Engineering heart tissues (EHTs) have emerged as a promising biomaterial to repair damaged heart. Nevertheless, selecting optimal candidate cells for EHTs has been controversial. Aging is a major consideration for seed cells derived from elderly patients. Hence, this study was aimed to assess the proliferation of, antiapoptosis potential of, and expression of senescence-associated factors (eg, SA-β-Gal, cyclin-dependent kinase inhibitor 2A (P16), cyclin-dependent kinase inhibitor 1 (P21) in adipose-derived stem cells (ADSCs), and bone marrow stem cells (BMSCs) in vitro. In addition, cardiac function, cell survival, and angiogenesis of ADSCs and BMSCs after SVR were assessed in vivo. The in vitro results showed that old ADSCs (OAs) grew faster; expressed lower levels of SA-β-Gal, P16, and P21; and possessed more pronounced antiapoptosis activity than old BMSCs (OBs). The in vivo results demonstrated that 28 days after patch implantation, animals that received OAs patches showed better restoration of cardiac function than animals that received OBs patches. Meanwhile, old ADSCs possessed more potential regarding cell survival and angiogenesis. These results suggest that ADSCs may be superior to BMSCs with regard to autologous cell transplantation in elderly patients.