Overexpression of Bop3 confers resistance to methylmercury in Saccharomyces cerevisiae through interaction with other proteins such as Fkh1, Rts1, and Msn2
Overexpression of Bop3 confers resistance to methylmercury in Saccharomyces cerevisiae through interaction with other proteins such as Fkh1, Rts1, and Msn2
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DOI：
10.1016/j.bbrc.2005.02.169
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发表时间：
2005-05-06
影响因子：
3.1
通讯作者：
Naganuma, A
中科院分区：
文献类型：
--
作者：
Hwang, GW;Furuoya, Y;Naganuma, A
我们发现，Bop 3，一种功能未知的蛋白质的过表达，赋予耐甲基汞的酿酒酵母。Bmh2、Fkh1和Rts1是先前通过双杂交方法显示与Bop 3结合的蛋白质。Bmh2和同源蛋白Bmh1的过表达赋予酵母对甲基汞的抗性，但Fkh1或Rts1的过表达具有最小的影响。然而，与野生型菌株相比，在Fhk1缺失的酵母中，Bop3过表达产生的对甲基汞的抗性的增加水平较小。相反，在Rts1缺失的酵母中，抗性程度显著升高。Msn2和Msn4先前被报道为与Bmh1和Bmh2结合的蛋白质。Msn2的过表达赋予了更大的敏感性，以甲基汞在酵母中，而相应的基因的缺失降低了程度的抗甲基汞诱导过表达的Bop3。这些结果表明，多个蛋白质参与最大限度地减少甲基汞的毒性诱导过表达的Bop3。(c)2005年爱思唯尔公司All rights reserved.
We found that overexpression of Bop3, a protein of unknown function, confers resistance to methylmercury in Saccharomyces cerevisiae. Bmh2, Fkh1, and Rts1 are proteins that have been previously shown to bind Bop3 by the two-hybrid method. Overexpression of Bmh2 and the homologous protein Bmh1 confers resistance to methylmercury in yeast, but overexpression of either Fkh1 or Rts1 has a minimal effect. However, the increased level of resistance to methylmercury produced by overexpression of Bop3 was smaller in Fhk1-deleted yeast as compared with that of the wild-type strain. In contrast, the degree of resistance was significantly elevated in Rts1-deleted yeast. Msn2 and Msn4 were previously reported as proteins that bind to Bmh1 and Bmh2. Overexpression of Msn2 conferred a much greater sensitivity to methylmercury in yeast, while deletion of the corresponding gene lowered the degree of resistance to methylmercury induced by overexpression of Bop3. These results suggest that multiple proteins are involved in minimizing the toxicity of methylmercury induced by overexpression of Bop3. (c) 2005 Elsevier Inc. All rights reserved.