Organosolvent pretreatment and enzymatic hydrolysis of rice straw for the production of bioethanol
Organosolvent pretreatment and enzymatic hydrolysis of rice straw for the production of bioethanol
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DOI：
10.1007/s11274-011-0838-8
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发表时间：
2012-02-01
影响因子：
4.1
通讯作者：
Pandey, Ashok
中科院分区：
文献类型：
--
作者：
Sindhu, Raveendran;Binod, Parameswaran;Pandey, Ashok
研究了有机溶剂预处理和水解稻草的工艺条件。在测试的不同有机酸和有机溶剂中，基于可发酵糖产率，发现丙酮是最有效的。对丙酮预处理工艺参数进行了优化。通过扫描电镜、X射线衍射和傅里叶变换红外光谱分析研究了预处理前后的结构变化。X-射线衍射分析表明，丙酮预处理的生物质的结晶度高于天然的。FTIR光谱也表现出显着差异之间的天然和预处理的样品。在最佳预处理条件下，每克预处理生物质产生0.458 g还原糖，发酵效率为39%。采用Box-Benhken设计对水解工艺参数进行优化，如生物量加载量、酶加载量、表面活性剂浓度和孵育时间。结果表明，丙酮预处理的稻草可以作为生物乙醇生产的良好原料。
The present study investigates the operational conditions for organosolvent pretreatment and hydrolysis of rice straw. Among the different organic acids and organic solvents tested, acetone was found to be most effective based on the fermentable sugar yield. Optimization of process parameters for acetone pretreatment were carried out. The structural changes before and after pretreatment were investigated by scanning electron microscopy, X-ray diffraction and Fourier transform infrared (FTIR) analysis. The X-ray diffraction profile showed that the degree of crystallinity was higher for acetone pretreated biomass than that of the native. FTIR spectrum also exhibited significant difference between the native and pretreated samples. Under optimum pretreatment conditions 0.458 g of reducing sugar was produced per gram of pretreated biomass with a fermentation efficiency of 39%. Optimization of process parameters for hydrolysis such as biomass loading, enzyme loading, surfactant concentration and incubation time was done using Box-Benhken design. The results indicate that acetone pretreated rice straw can be used as a good feed stock for bioethanol production.