Meiotic recombination-related DNA synthesis and its implications for cross-over and non-cross-over recombinant formation
Meiotic recombination-related DNA synthesis and its implications for cross-over and non-cross-over recombinant formation
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DOI：
10.1073/pnas.0611490104
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发表时间：
2007-04-03
影响因子：
11.1
通讯作者：
Ogawa, Tomoko
中科院分区：
文献类型：
--
作者：
Terasawa, Masahiro;Ogawa, Hideyuki;Ogawa, Tomoko
通过将胸苷类似物特异性定时掺入染色体中，分析了酿酒酵母中减数分裂重组相关的DNA合成（MRDS）。相对于已知的重组热点沿着DNA，掺入道的长度和位置被确定，掺入的时间和位置相对于形成和形成的联会复合体在伸展染色体中。不同的模式可以具体地与大多数的交叉和非交叉重组过程。所获得的结果提供了直接的证据，目前的共识重组模型的关键方面，提供信息的时间和空间之间的关系，非交叉形成和联会复合体，并提出的可能性，去除RecA同源Rad51起着关键作用，在调节发病的MRDS。最后，在果蝇，小鼠和百合的MRDS的经典观察很容易映射到这里提出的研究结果，提供了广泛保守的减数分裂重组过程的进一步证据。
Meiotic recombination-related DNA synthesis (MRDS) was analyzed in Saccharomyces cerevisiae by specifically timed incorporation of thymidine analogs into chromosomes. Lengths and positions of incorporation tracts were determined relative to a known recombination hot spot along DNA, as was the timing and localization of incorporation relative to forming and formed synaptonemal complex in spread chromosomes. Distinct patterns could be specifically associated with the majority cross-over and non-cross-over recombination processes. The results obtained provide direct evidence for key aspects of current consensus recombination models, provide information regarding temporal and spatial relationships between non-cross-over formation and the synaptonemal complex, and raise the possibility that removal of RecA homolog Rad51 plays a key role in regulating onset of MRDS. Finally, classical observations on MRDS in Drosophila, mouse, and lily are readily mapped onto the findings presented here, providing further evidence for a broadly conserved meiotic recombination process.