Advances in Mass Spectrometry for Lipidomics
Advances in Mass Spectrometry for Lipidomics
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DOI：
10.1146/annurev.anchem.111808.073705
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Mitchell, Todd W.
中科院分区：
文献类型：
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作者：
Blanksby, Stephen J.;Mitchell, Todd W.
最近在脂质组学领域的研究扩展是由新的质谱工具和方案的发展驱动的，用于鉴定和定量复杂基质中的分子脂质。尽管脂质组学领域与相关的质谱分析领域（如蛋白质组学）之间存在相似之处，但脂质在质谱分析中具有一些独特的优势和挑战。电喷雾电离对粗脂萃取物的应用，即所谓的散弹枪方法，就是这样一个例子，因为它在脂质组学中的应用可能比在任何其他学科中都要成功。相反，脂质分子结构的多样性意味着碰撞诱导解离在提供复杂脂质结构的独特描述方面可能受到限制，并且需要开发额外的、互补的离子激活和分析工具来克服这些挑战。在本文中，我们讨论了脂质谱技术的现状，并强调了当前方法不足和需要进一步创新的几个领域。
Recent expansion in research in the field of lipidomics has been driven by the development of new mass spectrometric tools and protocols for the identification and quantification of molecular lipids in complex matrices. Although there are similarities between the field of lipidomics and the allied field of mass spectrometry (e.g., proteomics), lipids present some unique advantages and challenges for mass spectrometric analysis. The application of electrospray ionization to crude lipid extracts without prior fractionation-the so-called shotgun approach-is one such example, as it has perhaps been more successfully applied in lipidomics than in any other discipline. Conversely, the diverse molecular structure of lipids means that collision-induced dissociation alone may be limited in providing unique descriptions of complex lipid structures, and the development of additional, complementary tools for ion activation and analysis is required to overcome these challenges. In this article, we discuss the state of the art in lipid mass spectrometry and highlight several areas in which current approaches are deficient and further innovation is required.