Structural, mechanical and in vitro characterization of individually structured Ti-6Al-4V produced by direct laser forming
Structural, mechanical and in vitro characterization of individually structured Ti-6Al-4V produced by direct laser forming
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DOI：
10.1016/j.biomaterials.2005.07.041
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发表时间：
2006-10-01
期刊：
影响因子：
14
通讯作者：
Erli, HJ
中科院分区：
文献类型：
--
作者：
Hollander, DA;von Walter, M;Erli, HJ
直接激光成形（DLF）是一种快速成型技术，它能够根据单个三维数据（包括解剖结构的计算机断层扫描模型）快速建模具有高体积密度的金属部件。在我们的项目中，我们在钛合金Ti-6Al-4V的基础上测试了DLF生产的材料作为硬组织生物材料的适用性。为此，我们研究了DLF-Ti-6Al-4V的力学和结构性能。虽然未处理的DLF合金的拉伸和屈服强度范围超过1000 MPa，但测定该材料的断裂伸长率为6.5 +/-0.6%。在额外的DLF后退火处理之后，该参数增加两倍至13.0 +/-0.6%，而拉伸强度和屈服强度降低了约10%。百分之八DLF退火后Ti-6Al-4V的杨氏模量为118.000 +/- 2.300 MPa。从DLF退火后Ti-6Al-4V的拉伸试验中获得的所有数据均符合美国材料与试验协会（ASTM）关于该合金用作医用材料的规范。旋转弯曲试验表明，DLF退火后Ti-6Al-4V的疲劳曲线与铸造/热等静压合金相当。我们通过扫描电子显微镜表征了未完成的DLF-Ti-6Al-4V的结构，并观察到颗粒的表面相关层，其可通过喷砂作为完成步骤来去除。我们制造了标称孔径为500、700和1000 μ m的多孔样品。直径通过所使用的DLF处理减小约10%。300亩在体外研究中，我们在无孔和多孔喷砂的DLF-Ti-6Al-4V标本上培养人成骨细胞，以研究细胞的形态、活力、增殖和分化。细胞在DLF-Ti-6Al-4V上铺展并增殖超过14天的培养时间。在多孔标本上，成骨细胞沿着孔的边缘生长，并形成圆形结构，如通过活/死染色以及扫描电子显微镜观察到的。总体而言，DLF-Ti-6Al-4V方法被证明是有效的，可以在硬组织生物材料领域进一步发展。(c)2005爱思唯尔有限公司保留所有权利。
Direct laser forming (DLF) is a rapid prototyping technique which enables prompt modelling of metal parts with high bulk density on the base of individual three-dimensional data, including computer tomography models of anatomical structures. In our project, we tested DLF-produced material on the basis of the titanium alloy Ti-6Al-4V for its applicability as hard tissue biomaterial. To this end, we investigated mechanical and structural properties of DLF-Ti-6Al-4V. While the tensile and yield strengths of untreated DLF alloy ranged beyond 1000 MPa, a breaking elongation of 6.5 +/- 0.6% was determined for this material. After an additional post-DLF annealing treatment, this parameter was increased two-fold to 13.0 +/- 0.6%, while tensile and yield strengths were reduced by approx. 8%. A Young's modulus of 118.000 +/- 2.300 MPa was determined for post-DLF annealed Ti-6Al-4V. All data gained from tensile testing of post-DLF annealed Ti-6Al-4V matched American Society of Testing and Materials (ASTM) specifications for the usage of this alloy as medical material. Rotating bending tests revealed that the fatigue profile of post-DLF annealed Ti-6Al-4V was comparable to casted/hot isostatic pressed alloy. We characterized the structure of non-finished DLF-Ti-6Al-4V by scanning electron microscopy and observed a surface-associated layer of particles, which was removable by sandblasting as a finishing step. We manufactured porous specimens with nominal pore diameters of 500, 700 and 1000 mu m. The diameters were reduced by the used DLF processing by approx. 300 mu m. In an in vitro investigation, we cultured human osteoblasts on non-porous and porous blasted DLF-Ti-6Al-4V specimens to study morphology, vitality, proliferation and differentiation of the cells. The cells spreaded and proliferated on DLF-Ti-6Al-4V over a culture time of 14 days. On porous specimens, osteoblasts grew along the rims of the pores and formed circle-shaped structures, as Visualized by live/dead staining as well as scanning electron microscopy. Overall, the DLF-Ti-6Al-4V approach proved to be efficient and could be further advanced in the field of hard tissue biomaterials. (c) 2005 Elsevier Ltd. All rights reserved.