Evaluation of the effects of various culture conditions on Cr(VI) reduction by Shewanella oneidensis MR-1 in a novel high-throughput mini-bioreactor
Evaluation of the effects of various culture conditions on Cr(VI) reduction by Shewanella oneidensis MR-1 in a novel high-throughput mini-bioreactor
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DOI：
10.1002/bit.21002
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发表时间：
2006-09-05
影响因子：
3.8
通讯作者：
Keasling, Jay D.
中科院分区：
文献类型：
--
作者：
Tang, Yinjie J.;Laidlaw, David;Keasling, Jay D.
使用微型生物反应器系统，独立监测和控制pH值溶解氧（DO），和温度为每个24：10-mL反应器的生长和Cr（VI）减少oneidensis MR-1进行了检查。盒中每个反应器的独立监测和控制允许探索已知影响S的环境条件的矩阵。oneidensis铬还原。S. oneidensis MR-1在需氧条件下生长在没有氨基酸或维生素补充的基本培养基中，但在厌氧条件下需要丝氨酸和甘氨酸补充。DO浓度> 80%时生长受到抑制。在对数生长期，低浓度的DO促进了乳酸向乙酸的转化。在11 - 35 ℃之间，生长速率服从Arrhenius反应速率-温度关系，最大生长速率发生在35 ℃。S. oneidensis MR-1能够在宽范围的pH（6-9）上生长。在中性pH和30至35摄氏度的温度范围内，S.在指数生长期，oneidensis MR-1在20分钟内将100 μ M Cr（VI）还原为Cr（III），并且生长速率不受铬酸盐添加的影响;在35 - 39 ℃的温度下，它甚至更快地还原铬酸盐。在低温（< 25 ℃）、酸性（pH < 6.5）或碱性（pH > 8.5）条件下，100 μ M Cr（VI）强烈抑制生长和铬酸盐还原。微型生物反应器系统能够快速测定这些参数，重复性和容易地进行很少的实验。除了用于检查环境修复的相关参数外，该设备还可以快速评估重组蛋白或次级代谢物的最佳生产参数。(c)2006 Wiley Periodicals，Inc.
The growth and Cr(VI) reduction by Shewanella oneidensis MR-1 was examined using a mini-bioreactor system that independently monitors and controls pH dissolved oxygen (DO), and temperature for each of its 24: 10-mL reactors. Independent monitoring and control of each reactor in the cassette allows the exploration of a matrix of environmental conditions known to influence S. oneidensis chromium reduction. S. oneidensis MR-1 grew in minimal medium without amino acid or vitamin supplementation under aerobic conditions but required serine and glycine supplementation under anaerobic conditions. Growth was inhibited by DO concentrations > 80%. Lactate transformation to acetate was enhanced by low concentration of DO during the logarithmic growth phase. Between 11 and 35 degrees C, the growth rate obeyed the Arrhenius reaction rate-temperature relationship, with a maximum growth rate occurring at 35 degrees C. S. oneidensis MR-1 was able to grow over a wide range of pH (6-9). At neutral pH and temperatures ranging from 30 to 35 degrees C, S. oneidensis MR-1 reduced 100 mu M Cr(VI) to Cr(III) within 20 min in the exponential growth phase, and the growth rate was not affected by the addition of chromate; it reduced chromate even faster at temperatures between 35 and 39 degrees C. At low temperatures (< 25 degrees C), acidic (pH < 6.5), or alkaline (pH > 8.5) conditions, 100 mu M Cr(VI) strongly inhibited growth and chromate reduction. The mini-bioreactor system enabled the rapid determination of these parameters reproducibly and easily by performing very few experiments. Besides its use for examining parameters of interest to environmental remediation, the device will also allow one to quickly assess parameters for optimal production of recombinant proteins or secondary metabolites. (c) 2006 Wiley Periodicals, Inc.