Ex Vivo Perfusion of the Rodent Placenta
Ex Vivo Perfusion of the Rodent Placenta
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DOI：
10.3791/59412
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发表时间：
2019-05-01
影响因子：
1.2
通讯作者：
Stapleton, Phoebe A.
中科院分区：
文献类型：
--
作者：
D'Errico, Jeanine N.;Fournier, Sara B.;Stapleton, Phoebe A.
胎盘是妊娠期间的一个关键器官，作为胎儿接触外源性物质的屏障，并介导营养物质与废物的交换。本文描述了一种体外灌流大鼠胎盘并评价外源性物质从母体到胎儿易位的试验。此外，可以用这种方法进行生理过程的评价，例如流向胎儿的液体和胎盘代谢。该技术适用于评价候选药物或环境污染物的母胎动力学。与目前的替代方法相比，该方法允许评价分离的母胎脉管系统，去除全身神经或免疫参与，允许任何观察到的生理功能变化归因于分离组织内的局部因素。
The placenta is a key organ during pregnancy that serves as a barrier to fetal xenobiotic exposure and mediates the exchange of nutrients for waste. An assay is described here to perfuse an isolated rat placenta and evaluate the maternal-to-fetal translocation of xenobiotics ex vivo. In addition, the evaluation of physiological processes such as fluid flow to the fetus and placental metabolism may be conducted with this methodology. This technique is suitable for evaluating maternal-to-fetal kinetics of pharmaceutical candidates or environmental contaminants. In contrast to current alternative approaches, this methodology allows the evaluation of the isolated maternal-fetal vasculature, with the systemic neural or immune involvement removed, allowing any observed changes in physiological function to be attributable to local factors within the isolated tissue.