The effect of different pine hosts on the sampling of Rhizopogon spore banks in five Eastern Sierra Nevada forests.
The effect of different pine hosts on the sampling of Rhizopogon spore banks in five Eastern Sierra Nevada forests.
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DOI：
10.1111/j.1469-8137.2006.01689.x
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发表时间：
2006-05
期刊：
影响因子：
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通讯作者：
T. Rusca;Peter G. Kennedy;T. Bruns
中科院分区：
文献类型：
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作者：
T. Rusca;Peter G. Kennedy;T. Bruns
我们的主要目标是确定是否检测到的Roppogon内转录间隔区（ITS）组的影响，在幼苗生物测定中使用的松树品种。我们的第二个目标是调查组成的Roppogon孢子银行在东部山脉内华达州的加州，以前未采样的地区。我们使用的幼苗扭叶松，松jeffreyi，兰伯松，和松muricata作为生物测定植物和内部转录间隔区（ITS）序列分析，确定检索到的Roppogon。我们发现，每种松树恢复了所有丰富的沙棘ITS组，但有显着差异的松树之间的丰富度沙棘ITS组恢复。结果表明，云南松恢复了本研究中发现的所有ITS类群，且恢复效果显著优于云南松。从5个采样点的Rpogon社区每个站点包含6至8个ITS组，有两个独特的序列组和更高的丰度Rpogon ellenae和Rpogon arctostaphyli组比以前采样的网站。这些结果表明，高的交叉感受性之间的Roppogon和松树种，和区域模式的孢子库组成。
Our primary goal was to determine whether detection of Rhizopogon internal transcribed spacer (ITS) groups is affected by the pine species used in seedling bioassays. Our secondary goal was to investigate composition of Rhizopogon spore banks in the Eastern Sierra Nevada of California, a previously unsampled region. We used seedlings of Pinus contorta, Pinus jeffreyi, Pinus lambertiana, and Pinus muricata as bioassay plants and identified the Rhizopogon retrieved by internal transcribed spacer (ITS) sequence analysis. We found that each of the pine species retrieved all of the abundant Rhizopogon ITS groups, but there were significant differences among pines in the richness of Rhizopogon ITS groups recovered. Pinus muricata recovered all ITS groups found in this study and was significantly better than P. lambertiana. Rhizopogon communities from the five sampled sites contained six to eight ITS groups per site, with two unique sequence groups and a higher abundance of the Rhizopogon ellenae and Rhizopogon arctostaphyli groups than at previously sampled sites. These results show high cross-receptivity between Rhizopogon and pine species, and regional patterns in spore bank composition.