A practical protocol for high-spatial-resolution magnetic resonance angiography for cerebral arteries in rats
A practical protocol for high-spatial-resolution magnetic resonance angiography for cerebral arteries in rats
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DOI：
10.1016/j.jneumeth.2023.109784
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发表时间：
2023-01-05
影响因子：
3
通讯作者：
Honmou，Osamu
中科院分区：
文献类型：
--
作者：
Nagahama，Hiroshi;Sasaki，Masanori;Honmou，Osamu
背景磁共振血管造影（MRA）是脑血管疾病大鼠模型的重要工具。尽管 MRA 长期以来一直用于啮齿动物，但图像质量通常不如临床实践中观察到的那么高。此外，对大鼠 MRA 图像质量的研究也很有限。本研究旨在开发一种实用的高空间分辨率MRA协议，用于大鼠脑动脉成像。新方法我们使用线圈放置的“半位法”，并修改了成像参数和图像重建方法。我们应用这种新的成像方法来测量大鼠 MRA 上与成熟相关的信号变化。结果只需修改线圈放置方法，新的实用高空间分辨率 MRA 成像协议即可获得高达使用基本线圈系统获得的信号强度的 3.5 倍。该方法可以检测到脑血管随着成熟而逐渐减少的信号。与现有方法相比，获得了大鼠的高空间分辨率 MRA，成像时间约为 100 分钟。使用新协议在 30 分钟的成像时间下获得了可比的分辨率和图像质量结论新的实用高空间分辨率 MRA 协议可以简单而成功地实现，以大约 30 分钟的成像时间实现高图像质量。该协议将有利于研究人员在大鼠脑动脉研究中进行 MRA 成像。
BackgroundMagnetic resonance angiography (MRA) is an important tool in rat models of cerebrovascular disease. Although MRA has long been used in rodents, the image quality is typically not as high as that observed in clinical practice. Moreover, studies on MRA image quality in rats are limited. This study aimed to develop a practical high-spatial-resolution MRA protocol for imaging cerebral arteries in rats.New methodWe used the “half position method” regarding coil placement and modified the imaging parameters and image reconstruction method. We applied this new imaging method to measure maturation-related signal changes on rat MRAs.ResultsThe new practical high-spatial-resolution MRA imaging protocol obtained a signal intensity up to 3.5 times that obtained using a basic coil system, simply by modifying the coil placement method. This method allowed the detection of a gradual decrease in the signal in cerebral vessels with maturation.Comparison with existing methodsA high-spatial-resolution MRA for rats was obtained with an imaging time of approximately 100 min. Comparable resolution and image quality were obtained using the new protocol with an imaging time of 30 minConclusionsThe new practical high-spatial-resolution MRA protocol can be implemented simply and successfully to achieve high image quality with an imaging time of approximately 30 min. This protocol will benefit researchers performing MRA imaging in cerebral artery studies in rats.