Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China.
Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China.
复制标题
27个位点的6种染料分型系统的开发验证及其在中国汉族人群中的应用
DOI：
10.1038/s41598-017-04548-1
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发表时间：
2017-07-05
影响因子：
4.6
通讯作者：
Ma S
中科院分区：
文献类型：
--
作者：
Liu Y;Guo L;Jin H;Li Z;Bai R;Shi M;Ma S
在这项研究中，一个新的27位点系统，（现称为SureID PanGlobal系统），包括24个常染色体STR（D3S1358、TH 01、D21S11、D18S51、Penta E、D12S391、D6S1043、D2S1338、D1S1656、D2S441、D5S818、D13S317、D7S820、D19S433、CSF1PO、Penta D、vWA、D8S1179、TPOX、FGA、D16 S539、D22 S1045、SE 33、D10 S1248）、两个Y染色体标记（DYS 391和Y-indel）和性别决定标记Amelogenin。所选STR基因座均为联合DNA索引系统（CODIS）和欧洲标准集（ESS）中的核心基因座，也有一些在商品化试剂盒和国家DNA数据库中常用的附加基因座。本文描述了使用Applied Biosystems 3500 XL基因分析仪对SureID PanGlobal系统进行的片段长度检测验证研究，包括以下参数和方面的分析：PCR条件、灵敏度、种属特异性、抑制、精密度、断续性、DNA混合物和犯罪现场样本的稳定性研究。研究表明，SureID PanGlobal系统对于法医应用和数据库具有可重复性、准确性、灵敏性和鲁棒性。此外，该系统的整个循环时间可以在65分钟内完成，这是专门为快速可靠地生成从血液，口腔拭子和法医染色获得的DNA图谱而开发的。
In this study, a novel 27-locus system (now known as the SureID PanGlobal system), including 24 autosomal STRs (D3S1358, TH01, D21S11, D18S51, Penta E, D12S391, D6S1043, D2S1338, D1S1656, D2S441, D5S818, D13S317, D7S820, D19S433, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D16S539, D22S1045, SE33, D10S1248), two Y-chromosome markers (DYS391 and Y-indel) and the sex determining marker, Amelogenin was developed with six fluorescent dyes labeling. The included STR loci belonged to the core loci in the Combined DNA Index System (CODIS) and the European Standard Set (ESS) as well as some additional loci commonly used in commercial kits and national DNA databases. This paper describes the validation studies conducted with the SureID PanGlobal system using Applied Biosystems 3500 XL Genetic Analyzer for fragment length detection that included the analysis of the following parameters and aspects: PCR conditions, sensitivity, species specificity, inhibition, precision, stutter, DNA mixtures, and stability studies with crime scene samples. The studies demonstrated that the SureID PanGlobal system is reproducible, accurate, sensitive and robust for forensic application and databasing. Additionally, the whole cycling time of the system can finish within 65 minutes, which was developed specifically for rapid and reliable generation of DNA profiles obtained from blood, buccal swabs and forensic stains.