Bonamia perspora n. sp (Haplosporidia), a Parasite of the Oyster Ostreola equestris, is the First Bonamia Species Known to Produce Spores
Bonamia perspora n. sp (Haplosporidia), a Parasite of the Oyster Ostreola equestris, is the First Bonamia Species Known to Produce Spores
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DOI：
10.1111/j.1550-7408.2006.00100.x
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发表时间：
2006-07-01
影响因子：
2.2
通讯作者：
Peterson, Charles H.
中科院分区：
文献类型：
--
作者：
Carnegie, Ryan B.;Burreson, Eugene M.;Peterson, Charles H.
对美国北卡罗来纳州里亚克河牡蛎(Crassostrea Ariakensis)中发现的一种波纳米亚(Bonamia)的潜在水库宿主--欧洲牡蛎(Ostreola Equestris)进行了研究，发现了第二个新的波纳米亚种(Bonamia sp.)。组织病理学、电子显微镜和分子系统发育分析支持将一个新的寄生虫种命名为Bonamia Perspora n.sp.，这是第一个被证明产生典型的单孢子虫孢子的波纳米亚种。经荧光原位杂交鉴定，证实孢子来自全孢子菌。在莫尔黑德市和北卡罗来纳州威尔明顿发现了多孢博纳米亚，总患病率为1.4%(31/2,144)。单核期、质原期和造孢子期几乎只出现在结缔组织中；单核期(2-6微米)在血细胞中很少观察到。孢子长4.3~6.4微米。在超微结构上，单核期、双核期和原质期与其他单孢子虫相似，但单孢子体较少，原质团较小。孢子纹饰由孢子壁衍生的薄而平的丝带组成，这些丝带随意地出现在孢子周围，并终止于似乎是四叉帽的地方。每个孢子的条带数在15~30条之间，长度在1.0~3.4亩之间。简约分析表明，全孢子芽孢杆菌是平菇的姊妹种。
Examination of the oyster Ostreola equestris as a potential reservoir host for a species of Bonamia discovered in Crassostrea ariakensis in North Carolina (NC), USA, revealed a second novel Bonamia sp. Histopathology, electron microscopy, and molecular phylogenetic analysis support the designation of a new parasite species, Bonamia perspora n. sp., which is the first Bonamia species shown to produce a typical haplosporidian spore with an orifice and hinged operculum. Spores were confirmed to be from B. perspora by fluorescent in situ hybridization. Bonamia perspora was found at Morehead City and Wilmington, NC, with an overall prevalence of 1.4% (31/2,144). Uninucleate, plasmodial, and sporogonic stages occurred almost exclusively in connective tissues; uninucleate stages (2-6 mu m) were rarely observed in hemocytes. Spores were 4.3-6.4 mu m in length. Ultrastructurally, uninucleate, diplokaryotic, and plasmodial stages resembled those of other spore-forming haplosporidians, but few haplosporosomes were present, and plasmodia were small. Spore ornamentation consisted of spore wall-derived, thin, flat ribbons that emerged haphazardly around the spore, and which terminated in what appeared to be four-pronged caps. Number of ribbons per spore ranged from 15 to 30, and their length ranged from 1.0 to 3.4 mu m. Parsimony analysis identified B. perspora as a sister species to Bonamia ostreae.