Bioactive potential of Streptomyces associated with marine sponges
Bioactive potential of Streptomyces associated with marine sponges
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DOI：
10.1007/s11274-009-0096-1
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发表时间：
2009-11-01
影响因子：
4.1
通讯作者：
Sumantha, Alagarsamy
中科院分区：
文献类型：
--
作者：
Dharmaraj, Selvakumar;Sumantha, Alagarsamy
本工作涉及与海洋海绵相关的链霉菌的分离及其生物活性。链霉菌属分离自海洋海绵海绵Callyspgia ffusa、Mycale mytilorum、Tedania anhelans和Dyside a Fragilis。初步筛选得到94株链霉菌，其中58株对细菌有拮抗作用，36株对真菌有拮抗作用，27株对两者都有广谱活性。对58株抗菌菌株的深层培养提取物进行了分析，其中37株对枯草芽孢杆菌、43株对金黄色葡萄球菌、10株对霍乱弧菌和10株对大肠杆菌呈阳性抑制。其中27株对黑曲霉、23株对酿酒酵母、16株对白色念珠菌有明显的抑制作用。从225~262 nm的吸收峰和0.40~0.78的TLC(R-f值)证实了活性提取物中多烯类物质的产生。结果表明，从海绵中分离的链霉菌菌株产生潜在的抗菌、抗真菌和广谱抗生素化合物。
The present work deals with isolation of Streptomyces associated with marine sponges and its bioactive potential. Streptomyces sp. were isolated from the marine sponges Callyspongia diffusa, Mycale mytilorum, Tedania anhelans and Dysidea fragilis. From the initial screening, 94 cultures of Streptomyces were obtained and from these 58 cultures exhibited antagonism against bacteria, 36 strains against fungi and 27 strains exhibited broad spectrum activity against both. The submerged culture extracts of the 58 anti-bacterial isolates were analysed and of these 58 strains, 37 strains showed positive inhibition against Bacillus subtilis, 43 against Staphylococcus aureus, 10 against Vibrio cholerae and 10 against Escherichia coli. The antifungal activities of the 36 strains were also evaluated and 27 strains showed positive inhibition against Aspergillus niger, 23 against Saccharomyces cerevisiae and 16 against Candida albicans. The production of polyene substances from the active extracts was confirmed by UV spectral analysis by the absorbance peaks that ranged from 225 to 262 nm and the TLC (R-f values) ranging from 0.40 to 0.78. The results indicate that Streptomyces strains isolated from marine sponges produce potential antibacterial, antifungal and broad spectrum antibiotic compounds.