Effect of FSH on E-2/GPR30-mediated mouse oocyte maturation in vitro
Effect of FSH on E-2/GPR30-mediated mouse oocyte maturation in vitro
复制标题
FSH对E-2/GPR30介导的小鼠卵母细胞体外成熟的影响
DOI：
10.1016/j.cellsig.2019.109464
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发表时间：
2020
影响因子：
4.8
通讯作者：
Ma Baohua
中科院分区：
文献类型：
--
作者：
Zhao Hui;Ge Junbang;Wei Juncai;Liu Jie;Liu Chen;Ma Chiyuan;Zhao Xiaoe;Wei Qiang;Ma Baohua
Mammalian oocyte restores meiosis can be stimulated by follicle-stimulating hormone (FSH) under normal physiological conditions. G-protein coupled receptor 30 (GPR30), an non-classical estrogen membrane receptor, has been widely reported in teleost oocyte maturation. However, it remains unknown whether GPR30 involves the role of FSH in mammalian cumulus expansion and oocyte maturation. Here, we used mouse cumulus-oocyte complexes (COCs) as a model to investigate how FSH affects the in vitro maturation of mouse oocytes mediated by 17β-estradiol (E2)/GPR30 signaling. Our study reveals that FSH starts regulating mouse cumulus expansion precisely at 8 h in in vitro culture. ELISA measurement of E2 levels in culture medium revealed that FSH activated aromatase to promote E2 production in vitro in cultured mouse COCs. Moreover, the results of real-time quantitative PCR indicated that FSH-induced in vitro maturation of mouse oocytes was regulated by the estrogensignaling pathway mediated by GPR30; FSH treatment markedly increased the mRNA expression of HAS2, PTGS2, and GREM1 in COCs. Exploration of the underlying mechanism suggested that E2 produced by mouse COCs regulated the phosphorylation level of extracellular signal-regulated kinase 1/2 (ERK1/2) through GPR30 and thereby promoted mouse cumulus-cell expansion and oocyte maturation. In conclusion, our study reveals that FSH induced estrogen production in mouse COCs through aromatase, and that aromatase/GPR30/ERK1/2 signaling is involved in FSH-induced cumulus expansion.
